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Product Info Summary
| SKU: | M09159 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Hydroxyacid oxidase 1 HAO1 Monoclonal Antibody
SKU/Catalog Number
M09159
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Hydroxyacid oxidase 1 HAO1 Monoclonal Antibody catalog # M09159. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hydroxyacid oxidase 1 HAO1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09159)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
Mix
Isotype
IgG
Immunogen
Recombinant Protein of HAO1
Reactive Species
M09159 is reactive to HAO1 in Mouse, Rat
Calculated molecular weight
40.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09159 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-2000
IF 1:200
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.
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Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, HAO1 Monoclonal Antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, Chk2 (phospho Thr68) Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). HAO1 Monoclonal Antibody (Mix) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Immunofluorescence analysis of Mouse-spleen tissue. 1, HAO1 Monoclonal Antibody (Mix) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Specific Publications For Anti-Hydroxyacid oxidase 1 HAO1 Monoclonal Antibody (M09159)
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