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Product Info Summary
| SKU: | M04351 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CITED1 Mouse Monoclonal Antibody [Clone ID: OTI3C12]
SKU/Catalog Number
M04351
Size
100 µl
Description
Boster Bio CITED1 mouse monoclonal antibody, clone OTI3C12 (formerly 3C12). Catalog# M04351. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CITED1 Mouse Monoclonal Antibody [Clone ID: OTI3C12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04351)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3C12
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CITED1 (NP_004134) produced in E.coli.
Reactive Species
M04351 is reactive to CITED1 in Human
Calculated molecular weight
19.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04351 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CITED1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CITED1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CITED1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-CITED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Specific Publications For Anti-CITED1 Mouse Monoclonal Antibody [Clone ID: OTI3C12] (M04351)
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