Product Info Summary
SKU: | M02539 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-LOXL2 Rabbit Monoclonal Antibody
View all Lysyl Oxidase Homolog 2/LOXL2 Antibodies
SKU/Catalog Number
M02539
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LOXL2 Rabbit Monoclonal Antibody catalog # M02539. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LOXL2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02539)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBD-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LOXL2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02539 is reactive to LOXL2 in Human, Mouse, Rat
Applications
M02539 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
23 kDa
Calculated molecular weight
86.725kDa
Background of Lysyl Oxidase Homolog 2/LOXL2
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of LOXL2 expression in MCF7 cell lysate.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For LOXL2 (Source: Uniprot.org, NCBI)
Gene Name
LOXL2
Full Name
Lysyl oxidase homolog 2
Weight
86.725kDa
Superfamily
lysyl oxidase family
Alternative Names
EC 1.4.3; EC 1.4.3.-; LOL2; LOR2; LOXL2; Lysyl Oxidase Homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; WS9-14 LOXL2 LOR, LOR2, WS9-14 lysyl oxidase like 2 lysyl oxidase homolog 2|lysyl oxidase related 2|lysyl oxidase-like 2 delta e13|lysyl oxidase-like 2 protein|lysyl oxidase-like protein 2|lysyl oxidase-related protein 2|lysyl oxidase-related protein WS9-14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LOXL2, check out the LOXL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LOXL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LOXL2 Rabbit Monoclonal Antibody (M02539)
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No publications found for M02539
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-LOXL2 Rabbit Monoclonal Antibody
Question
Does M02539 anti-LOXL2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can see on the product datasheet, M02539 anti-LOXL2 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-25
Question
What sample target on Western Blot application was tested with M02539?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of LOXL2 expression in MCF7 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
My question regards to test anti-LOXL2 Monoclonal antibody M02539 on human adipose tissue for research purposes, then I may be interested in using anti-LOXL2 Monoclonal antibody M02539 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The products we sell, including anti-LOXL2 Monoclonal antibody M02539, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-24
Question
I have a question about product M02539, anti-LOXL2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Rodriguez
Verified customer
Asked: 2018-05-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02539 anti-LOXL2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-24
Question
Will anti-LOXL2 Monoclonal antibody M02539 work for IF with adipose tissue?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
According to the expression profile of adipose tissue, LOXL2 is highly expressed in adipose tissue. So, it is likely that anti-LOXL2 Monoclonal antibody M02539 will work for IF with adipose tissue.
Boster Scientific Support
Answered: 2017-10-23