Click image to see more details
Product Info Summary
| SKU: | A08090 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cleaved-MMP-17 (Q129) Antibody
SKU/Catalog Number
A08090
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-MMP-17 (Q129) Antibody catalog # A08090. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-MMP-17 (Q129) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08090)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP17. AA range:110-159
Reactive Species
A08090 is reactive to MMP17 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
66.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08090 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using Cleaved-MMP-17 (Q129) Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 1h, using MMP17 (Cleaved-Gln129) Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cleaved-MMP-17 (Q129) Antibody (A08090)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cleaved-MMP-17 (Q129) Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Cleaved-MMP-17 (Q129) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Cleaved-MMP-17 (Q129) Antibody
Question
Would anti-Cleaved-MMP-17 (Q129) antibody A08090 work for ELISA with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
According to the expression profile of mammary carcinoma, MMP17 is highly expressed in mammary carcinoma. So, it is likely that anti-Cleaved-MMP-17 (Q129) antibody A08090 will work for ELISA with mammary carcinoma.
Boster Scientific Support
Answered: 2020-03-11
Question
Does A08090 anti-Cleaved-MMP-17 (Q129) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
It shows on the product datasheet, A08090 anti-Cleaved-MMP-17 (Q129) antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-02
Question
Can you help my question with product A08090, anti-Cleaved-MMP-17 (Q129) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jha
Verified customer
Asked: 2019-07-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08090 anti-Cleaved-MMP-17 (Q129) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-01
Question
Will anti-Cleaved-MMP-17 (Q129) antibody A08090 work on goat ELISA with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
Our lab technicians have not validated anti-Cleaved-MMP-17 (Q129) antibody A08090 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Cleaved-MMP-17 (Q129) antibody A08090 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat mammary carcinoma in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-11
Question
We are currently using anti-Cleaved-MMP-17 (Q129) antibody A08090 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
The anti-Cleaved-MMP-17 (Q129) antibody (A08090) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-Cleaved-MMP-17 (Q129) antibody A08090. Let me know if you need anything else.
G. Williams
Verified customer
Asked: 2016-03-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-11
Question
I see that the anti-Cleaved-MMP-17 (Q129) antibody A08090 works with ELISA, what is the protocol used to produce the result images on the product page?
C. Thomas
Verified customer
Asked: 2015-01-09
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-01-09
