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Product Info Summary
| SKU: | A04554-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-COL9A1 Antibody (Center)
SKU/Catalog Number
A04554-2
Size
80 µl
Description
Boster Bio Anti-COL9A1 Antibody (Center) (Catalog # A04554-2). Tested in IHC-P, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-COL9A1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04554-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This COL9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-456 amino acids from the Central region of human COL9A1.
Reactive Species
A04554-2 is reactive to COL9A1 in Human, Mouse, Rat
Calculated molecular weight
91.9 kDa
Background of COL9A1
COL9A1 is one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule,and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in the COL9A1 gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04554-2 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-COL9A1 Antibody (Center) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 92 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with COL9A1 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-COL9A1 Antibody (Center) (A04554-2)
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