Click image to see more details
Product Info Summary
| SKU: | A00517-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Collagen II/COL2A1 Antibody Picoband®
SKU/Catalog Number
A00517-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen II/COL2A1 Antibody Picoband® catalog # A00517-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00517-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, which shares 91.7% and 95.8% amino acid (aa) sequence identity with mouse and rat Collagen II, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00517-1 is reactive to COL2A1 in Human, Mouse, Rat
Observed Molecular Weight
142 kDa
Calculated molecular weight
141.8 kDa
Background of COL2A1
Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00517-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat cartilage tissue, mouse cartilage tissue, mouse auricular cartilage
Validation Images & Assay Conditions
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Western blot analysis of COL2A1 using anti-COL2A1 antibody (A00517-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat cartilage tissue lysates,
Lane 2: mouse cartilage tissue lysates,
Lane 3: mouse auricular cartilage lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL2A1 antigen affinity purified polyclonal antibody (Catalog # A00517-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL2A1 at approximately 180-190 kDa. The expected band size for COL2A1 is at 142 kDa.
Specific Publications For Anti-Collagen II/COL2A1 Antibody Picoband® (A00517-1)
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Customer Q&As
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4 Customer Q&As for Anti-Collagen II/COL2A1 Antibody Picoband®
Question
We have been able to see staining in human fetal sternum. Are there any suggestions? Is anti-Collagen II/COL2A1 antibody supposed to stain fetal sternum positively?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
From what I have seen in literature fetal sternum does express COL2A1. From what I have seen in Uniprot.org, COL2A1 is expressed in tibia, blood, embryonic stem cell muscle, fetal sternum, among other tissues. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2019-09-23
Question
I am interested in using your anti-Collagen II/COL2A1 antibody for skeletal system development studies. Has this antibody been tested with western blotting on rat liver tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We appreciate your inquiry. This A00517-1 anti-Collagen II/COL2A1 antibody is validated on rat liver tissue, mouse liver tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-13
Question
We are currently using anti-Collagen II/COL2A1 antibody A00517-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
The anti-Collagen II/COL2A1 antibody (A00517-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-21
Question
We were well pleased with the WB result of your anti-Collagen II/COL2A1 antibody. However we have been able to see positive staining in embryonic stem cell muscle extracellular using this antibody. Is that expected? Could you tell me where is COL2A1 supposed to be expressed?
H. Yang
Verified customer
Asked: 2014-10-15
Answer
Based on literature, embryonic stem cell muscle does express COL2A1. Generally COL2A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2014-10-15
