Anti-Collagen III/COL3A1 Antibody

Collagen III alpha 1/COL3A1 antibody

Boster Bio Anti-Collagen III/COL3A1 Antibody catalog # RP1033. Tested in WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: RP1033
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Collagen III/COL3A1 Antibody

View all Collagen III alpha 1/COL3A1 Antibodies

SKU/Catalog Number

RP1033

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen III/COL3A1 Antibody catalog # RP1033. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen III/COL3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1033)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III (1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1033 is reactive to COL3A1 in Human

Applications

RP1033 is guaranteed for WB Boster Guarantee

Background of Collagen III alpha 1/COL3A1

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For COL3A1 (Source: Uniprot.org, NCBI)

Uniprot ID

P02461

Gene ID

1281

Gene Name

COL3A1

Full Name

Collagen alpha-1(III) chain

Weight

138.564kDa

Superfamily

fibrillar collagen family

Alternative Names

alpha1 (III) collagen; COL3A1; Collagen 3; collagen alpha-1(III) chain; Collagen III alpha 1; collagen, fetal; collagen, type III, alpha 1; Collagen-3; EDS4A; Ehlers-Danlos syndrome type IV, autosomal dominant; FLJ34534 COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COL3A1, check out the COL3A1 Infographic

COL3A1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COL3A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

RP1033 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xu L,Sun N,Li G,Liu L.LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway.Mol Cell Biochem.2021 Jan 3.doi:10.1007/s11010-020-04024-x.Epub ahead of print.PMID:33389493.
Species: Human
RP1033 usage in article: APP:WB, SAMPLE:KELOID FIBROBLASTS, DILUTION:1:1000

BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway

Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.

Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target

Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats

Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice

Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation

Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis

Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

Astragalus membranaceus?ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition?in vivo?and?in vitro

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15 Customer Q&As for Anti-Collagen III/COL3A1 Antibody

Question

Would RP1033 anti-Collagen III/COL3A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

You can see on the product datasheet, RP1033 anti-Collagen III/COL3A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-13

Question

Do you have a BSA free version of anti-Collagen III/COL3A1 antibody RP1033 available?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen III/COL3A1 antibody RP1033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-31

Question

Our lab want to know about using your anti-Collagen III/COL3A1 antibody for response to cytokine studies. Has this antibody been tested with western blotting on colo320 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

Thanks for your inquiry. This RP1033 anti-Collagen III/COL3A1 antibody is validated on colo320 whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-18

Question

My question regarding product RP1033, anti-Collagen III/COL3A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1033 anti-Collagen III/COL3A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen III/COL3A1 antibody RP1033. Let me know if you need anything else.

N. Edwards

Verified customer

Asked: 2019-08-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-06

Question

My question regards to test anti-Collagen III/COL3A1 antibody RP1033 on human colon carcinoma for research purposes, then I may be interested in using anti-Collagen III/COL3A1 antibody RP1033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

The products we sell, including anti-Collagen III/COL3A1 antibody RP1033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-07

Question

Will anti-Collagen III/COL3A1 antibody RP1033 work for WB with colon carcinoma?

Verified Customer

Verified customer

Asked: 2019-05-20

Answer

According to the expression profile of colon carcinoma, COL3A1 is highly expressed in colon carcinoma. So, it is likely that anti-Collagen III/COL3A1 antibody RP1033 will work for WB with colon carcinoma.

Boster Scientific Support

Answered: 2019-05-20

Question

Is a blocking peptide available for product anti-Collagen III/COL3A1 antibody (RP1033)?

E. Patel

Verified customer

Asked: 2019-01-08

Answer

We do provide the blocking peptide for product anti-Collagen III/COL3A1 antibody (RP1033). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-08

Question

See attached the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen III/COL3A1 antibody RP1033. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-09-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-09-13

Question

I see that the anti-Collagen III/COL3A1 antibody RP1033 works with WB, what is the protocol used to produce the result images on the product page?

P. Wu

Verified customer

Asked: 2018-08-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-08-21

Question

We were well pleased with the WB result of your anti-Collagen III/COL3A1 antibody. However we have been able to see positive staining in uterine cervix secreted using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

According to literature, uterine cervix does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2018-02-01

Question

We are currently using anti-Collagen III/COL3A1 antibody RP1033 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-15

Answer

The anti-Collagen III/COL3A1 antibody (RP1033) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-15

Question

Is this RP1033 anti-Collagen III/COL3A1 antibody reactive to the isotypes of COL3A1?

Verified Customer

Verified customer

Asked: 2017-07-18

Answer

The immunogen of RP1033 anti-Collagen III/COL3A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III(1200-1221aa VGAAAIAGIGGEKAGGFAPYYG), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-07-18

Question

I was wanting to use your anti-Collagen III/COL3A1 antibody for WB for human colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon carcinoma identification?

L. Williams

Verified customer

Asked: 2017-05-04

Answer

You can see on the product datasheet, RP1033 anti-Collagen III/COL3A1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-05-04

Question

We have been able to see staining in human colon carcinoma. Are there any suggestions? Is anti-Collagen III/COL3A1 antibody supposed to stain colon carcinoma positively?

R. Miller

Verified customer

Asked: 2014-10-17

Answer

From what I have seen in literature colon carcinoma does express COL3A1. From what I have seen in Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886

Boster Scientific Support

Answered: 2014-10-17

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Total: $315

SKU:RP1033

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RP1033
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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