|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)|
|Description||Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) FH-7A (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)|
|Replacement Item||This antibody may replace the following items: sc-28888|sc-8781|sc-271249|sc-8780-R|sc-514601 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human collagen type III.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Collagen alpha-1(III) chain|
|Molecular Weight||138936 MW|
|Protein Function||Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .|
|Sequence Similarities||Belongs to the fibrillar collagen family.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix .|
|Alternative Names||Collagen alpha-1(III) chain;Col3a1;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|collagen| stem cells|mesenchymal stem cells|chondrogenesis|osteogenesis||
Background for Collagen alpha-1(III) chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, -|
Western blot, 0.25-0.5μg/ml, Human, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) Images
Click the images to enlarge.
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
IHC(F): Mouse Kidney Tissue