|Product Name||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)|
|Immunogen||Human collagen type III.|
Assay Dilutions Overview
Western blot, 0.25-0.5μg/ml, Human, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
Images And Assay Conditions
Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F)
IHC(F): Mouse Kidney Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Collagen alpha-1(III) chain|
|Alternative Names||Collagen alpha-1(III) chain;Col3a1;|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix .|
|Molecular Weight||138936 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .|
|Background||Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.|
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