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- Table of Contents
COL3A1 antibody (M00788) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IHC analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (M00788).
Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Collagen III/COL3A1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-Collagen III/COL3A1 Antibody (M00788) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9)
COL3A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, WB |
Tested Applications
COL3A1 antibody was positively detected by WB in:
human placenta tissue, Hela whole cell, rat heart tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
COL3A1 antibody was positively detected by IHC in:
human intestinal cancer tissue, human mammary cancer tissue, human intestinal cancer tissue, human lung cancerr tissue, mouse intestine tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9)
View all COL3A1 Antibodies
View all COL3A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
9H9
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222- E1455).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
180-190 kDa
Calculated Molecular Weight
138.6 kDa
Background of COL3A1
COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Cite This Product
Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00788)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Mouse
92%
High Homology
Rat
92%
High Homology
Dog
92%
High Homology
Zebrafish
66%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for COL3A1 antibody
Immunohistochemistry (IHC) protocol for COL3A1 antibody
Publications
Application:
Species:
Ruthenium Oxide Nanozyme for Tendinopathy Treatment via Countering Ferroptosis
Published: 2025-06-12
Journal: Advanced Healthcare Materials
Application: WB,IF
Impact Factor: 9.60
Species: Rat
PMID: 40509609
Combination Treatment of Timosaponin BII and Pirfenidone Attenuated Pulmonary Fibrosis Through Anti-Inflammatory and Anti-Fibrotic Process in Rodent Pulmonary Fibrosis Model and Cellular Epithelial–Mesenchymal Transition Model
Published: 2025-04-18
Journal: MOLECULES
Application: IHC
Impact Factor: 4.60
Species: Mouse
PMID: 40333870
3D bioprinting of a dermal scaffold for full-thickness skin tissue regeneration
Published: 2024-12-27
Journal: Bio-Design and Manufacturing
Application: IF
Impact Factor: 8.10
Species: Human
Exosomal miRNA reprogramming in pyroptotic macrophage drives silica-induced fibroblast-to-myofibroblast transition and pulmonary fibrosis
Published: 2024-11-22
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: WB
Impact Factor: 12.20
Species: Mouse
PMID: 39603130
Exosomes Derived from Antler Mesenchymal Stem Cells Promote Wound Healing by miR-21-5p/STAT3 Axis
Published: 2024-11-03
Journal: International Journal of Nanomedicine
Application: IHC
Impact Factor: 6.60
Species: Mouse
PMID: 39524924
Microrna363-5p targets thrombospondin3 to regulate pathological cardiac remodeling
Published: 2024-10-07
Journal: MOLECULAR AND CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.50
Species: Mouse,Human
PMID: 39373825
Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway
Published: 2024-04-24
Journal: Natural Products and Bioprospecting
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38656633
Involvement of oxidative stress-AMPK-Cx43-NLRP3 pathway in extracellular matrix remodeling of gastric smooth muscle cells in rats with diabetic gastroparesis
Published: 2024-04-21
Journal: CELL STRESS & CHAPERONES
Application: IHC
Impact Factor: 3.30
Species: Rat
PMID: 38653383
A heparin-functionalized bioink with sustained delivery of vascular endothelial growth factor for 3D bioprinting of prevascularized dermal constructs
Published: 2024-02-08
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IF
Impact Factor: 7.70
Species: Human
PMID: 38340924
Scutellarin ameliorates diabetic nephropathy via TGF-β1 signaling pathway
Published: 2023-10-17
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 2.00
Species: Mouse
PMID: 37853616
Radiation‑induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues
Published: 2021-10-11
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 2.95
Species: Mouse
PMID: 34633055
ASK1 Enhances Angiotensin II-Induced Liver Fibrosis In Vitro by Mediating Endoplasmic Reticulum Stress-Dependent Exosomes
Published: 2020-11-09
Journal: MEDIATORS OF INFLAMMATION
Application: WB
Impact Factor: 3.76
Species: Human
PMID: 33223956
Rapamycin attenuates the paraquat-induced pulmonary fibrosis through activating Nrf2 pathway
Published: 2019-07-12
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.52
Species: Rat
PMID: 31301076
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Product Promise
We promise Anti-Collagen III/COL3A1 Antibody Picoband® (monoclonal, 9H9) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00788
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026