Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Human collagen type III.|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, -
Western blot, 0.25-0.5μg/ml, Human, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
Images And Assay Conditions
Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F)
IHC(F): Mouse Kidney Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Collagen alpha-1(III) chain|
|Alternative Names||Collagen alpha-1(III) chain;Col3a1;|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix .|
|Molecular Weight||138936 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .|
|Research Areas||Human, Rat
*You can search these to find other products in these research areas.
|Background||Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Boster offers many ascites grade monoclonal antibodies that are sold at 100 microgram size. Does the 100 micrograms refer to amount of target-specific antibody? Keyword: concentrationA: MA1029 is lyophilized, and the lyophilized lump contain 100ug target-specific antibody and other protein as the agent for protein protection, the ratio is 1:20. The antibody is supplied as mouse ascites, it is not purified. We just detect it in WB.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact firstname.lastname@example.org
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to email@example.com.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to col3a1 antibody, type iii collagen antibody