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Product Info Summary
| SKU: | MA1029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)
SKU/Catalog Number
MA1029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A) catalog # MA1029. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
FH-7A
Isotype
Mouse IgG1
Immunogen
Human collagen type III.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1029 is reactive to Col3a1 in Human, Rat
Observed Molecular Weight
49 kDa
Calculated molecular weight
138.9 kDa
Background of Col3a1
Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1029 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, -
Western blot, 0.25-0.5μg/ml, Human, rat
Positive Control
WB: HT1080 Cell, COLO320 Cell
IHC-F: Mouse Kidney Tissue
Validation Images & Assay Conditions
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Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
Click image to see more details
Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F)
IHC(F): Mouse Kidney Tissue
Specific Publications For Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A) (MA1029)
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)
Question
We are currently using anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The anti-Collagen, Type III antibody (Monoclonal, FH-7A) (MA1029) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-02
Question
Does anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 work on goat IHC with skin fibroblast?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not tested anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin fibroblast in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029. Let me know if you need anything else.
S. Wu
Verified customer
Asked: 2019-12-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
Our lab were well pleased with the WB result of your anti-Collagen, Type III antibody (Monoclonal, FH-7A). However we have been able to see positive staining in placenta secreted using this antibody. Is that expected? Could you tell me where is COL3A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
From what I have seen in literature, placenta does express COL3A1. Generally COL3A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2019-11-28
Question
Does MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
It shows on the product datasheet, MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-20
Question
My question regarding product MA1029, anti-Collagen, Type III antibody (Monoclonal, FH-7A). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-27
Question
I am interested in to test anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 on human colon carcinoma for research purposes, then I may be interested in using anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The products we sell, including anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-26
Question
I see that the anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 works with WB, what is the protocol used to produce the result images on the product page?
A. Li
Verified customer
Asked: 2019-03-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-01
Question
Is a blocking peptide available for product anti-Collagen, Type III antibody (Monoclonal, FH-7A) (MA1029)?
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
We do provide the blocking peptide for product anti-Collagen, Type III antibody (Monoclonal, FH-7A) (MA1029). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-10
Question
Would anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 work for WB with colon carcinoma?
G. Mitchell
Verified customer
Asked: 2018-12-04
Answer
According to the expression profile of colon carcinoma, COL3A1 is highly expressed in colon carcinoma. So, it is likely that anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2018-12-04
Question
Boster offers many ascites grade monoclonal antibodies that are sold at 100 microgram size. Does the 100 micrograms refer to amount of target-specific antibody? Keyword: concentration
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
MA1029 is lyophilized, and the lyophilized lump contain 100ug target-specific antibody and other protein as the agent for protein protection, the ratio is 1:20. The antibody is supplied as mouse ascites, it is not purified. We just detect it in WB.
Boster Scientific Support
Answered: 2018-08-06
Question
We have observed staining in rat colon carcinoma. Are there any suggestions? Is anti-Collagen, Type III antibody (Monoclonal, FH-7A) supposed to stain colon carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
From what I have seen in literature colon carcinoma does express COL3A1. From what I have seen in Uniprot.org, COL3A1 is expressed in uterine cervix, skin fibroblast, liver, placenta, colon carcinoma, among other tissues. Regarding which tissues have COL3A1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 7864881
Liver, Pubmed ID: 15489334, 24275569
Placenta, Pubmed ID: 3405773
Skin fibroblast, Pubmed ID: 2764886
Boster Scientific Support
Answered: 2018-08-06
Question
Is this MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) reactive to the isotypes of COL3A1?
Verified Customer
Verified customer
Asked: 2018-05-10
Answer
The immunogen of MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) is Human collagen type III. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-10
Question
Please see the WB image, lot number and protocol we used for colon carcinoma using anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
I was wanting to use using your anti-Collagen, Type III antibody (Monoclonal, FH-7A) for cerebral cortex development studies. Has this antibody been tested with western blotting on ht1080 cell lysate? We would like to see some validation images before ordering.
V. Kulkarni
Verified customer
Asked: 2017-01-27
Answer
Thanks for your inquiry. This MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) is tested on ht1080 cell lysate, colo320 cell lysate, mouse kidney tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-27
Question
Our lab used your anti-Collagen, Type III antibody (Monoclonal, FH-7A) for WB on uterine cervix in a previous project. I am using human, and We want to use the antibody for IHC next. Our lab want to know about examining uterine cervix as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
H. Banerjee
Verified customer
Asked: 2016-10-19
Answer
I viewed the website and datasheets of our anti-Collagen, Type III antibody (Monoclonal, FH-7A) and it seems that MA1029 has been validated on human in both WB and IHC. Thus MA1029 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-10-19
Question
Do you have a BSA free version of anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 available?
G. Parker
Verified customer
Asked: 2015-05-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen, Type III antibody (Monoclonal, FH-7A) MA1029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-22
Question
I was wanting to use your anti-Collagen, Type III antibody (Monoclonal, FH-7A) for WB for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
C. Parker
Verified customer
Asked: 2013-11-08
Answer
As indicated on the product datasheet, MA1029 anti-Collagen, Type III antibody (Monoclonal, FH-7A) has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-08
