radio
SKU MA1029
Size 100μg/vial
Reactivity Human, Rat
Clonality Monoclonal
Clone Number FH-7A
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Collagen, Type III Antibody (Monoclonal, FH-7A)
SKU/Catalog Number MA1029
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Collagen, Type III, collagen, type III, alpha 1 (COL3A1) detection. Tested with WB, IHC-F in Human;rat. No cross reactivity with other proteins.
Cite This Product Anti-Collagen, Type III Antibody (Monoclonal, FH-7A) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1029)
Host Mouse
Immunogen Human collagen type III.
Reactivity Human, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, rat, -
Western blot, 0.25-0.5μg/ml, Human, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

/antibody/ma1029 1 WB anti collagen type iii antibody monoclonal fh 7a.jpg

Anti-Collagen, Type III antibody (monoclonal), MA1029, Western blotting
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate

/antibody/ma1029 2 IHC anti collagen type iii antibody monoclonal fh 7a.jpg

Anti-Collagen, Type III antibody (monoclonal), MA1029, IHC(F)
IHC(F): Mouse Kidney Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P13941
Gene Name Col3a1
Protein Name Collagen alpha-1(III) chain
Alternative Names Collagen alpha-1(III) chain;Col3a1;
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Molecular Weight 138936 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .
Background Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Order Product (MA1029)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-?1 with concomitant induce Smad7 in human hypertrophic scar fibroblasts
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
BMP-7 suppresses excessive scar formation by activating the BMP-7/Smad1/5/8 signaling pathway
Long-term intravenous administration of carboxylated single-walled carbon nanotubes induces persistent accumulation in the lungs and pulmonary fibrosis via the nuclear factor-kappa B pathway.
Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars
Role of transforming growth factor ?-1 in the pathogenesis of pelvic organ prolapse: A potential therapeutic target
Astragalus membranaceusameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transitionin vivoandin vitro
Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro
Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats
Continuing Treatment with Salvia miltiorrhiza Injection Attenuates Myocardial Fibrosis in Chronic Iron-Overloaded Mice
Over-Expression of Calpastatin Inhibits Calpain Activation and Attenuates Post-Infarction Myocardial Remodeling
CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages
Antiphotoaging Effect of Conditioned Medium of Dedifferentiated Adipocytes on Skin In Vivo and In Vitro: A Mechanistic Study
Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation
?-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/?-catenin signaling inhibits hepatic stellate cell activation
Localization of Human Mesenchymal Stem Cells from Umbilical Cord Blood and Their Role in Repair of Diabetic Foot Ulcers in Rats
Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells
Attenuation of portal hypertension by natural taurine in rats with liver cirrhosis
Effects of pentoxifylline on the hepatic content of TGF-?1 and collagen in Schistosomiasis japonica mice with liver fibrosis
Wang X, Cao P, Liu J, Du P, Wang Z, Chen W, Liu C, Wu Y. Med Sci Monit. 2017 Jan 4;23:46-56. 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts
Xu Y, Zhang Ja, Xu Y, Guo Sl, Wang S, Wu D, Wang Y, Luo D, Zhou Br. Stem Cells Dev. 2015 May 1;24(9):1096-111. Doi: 10.1089/Scd.2014.0321. Epub 2015 Jan 21. Antiphotoaging Effect Of Conditioned Medium Of Dedifferentiated Adipocytes On Skin In Vivo...
Liang J, Deng X, Lin Zx, Zhao Lc, Zhang Xl. World J Gastroenterol. 2009 Sep 28;15(36):4529-37. Attenuation Of Portal Hypertension By Natural Taurine In Rats With Liver Cirrhosis.
Ge Ws, Wu Jx, Fan Jg, Wang Yj, Chen Yw. World J Gastroenterol. 2011 Sep 28;17(36):4090-8. Doi: 10.3748/Wjg.V17.I36.4090. Inhibition Of High-Mobility Group Box 1 Expression By Sirna In Rat Hepatic Stellate Cells.
Zunwen L, Shizhen Z, Dewu L, Yungui M, Pu N. Burns. 2012 May;38(3):404-13. Doi: 10.1016/J.Burns.2011.08.013. Epub 2011 Sep 16. Effect Of Tetrandrine On The Tgf-??-Induced Smad Signal Transduction Pathway In Human Hypertrophic Scar Fibroblasts In Vi...
Li X, Han J, Li L, Wang Kj, Hu Sj. Int J Cardiol. 2013 Oct 9;168(4):3340-7. Doi: 10.1016/J.Ijcard.2013.04.038. Epub 2013 May 9. Effect Of Farnesyltransferase Inhibition On Cardiac Remodeling In Spontaneously Hypertensive Rats.
Han L, Wang L, Wang Q, Li H, Zang H. Exp Ther Med. 2014 May;7(5):1337-1341. Epub 2014 Feb 20. Association Between Pelvic Organ Prolapse And Stress Urinary Incontinence With Collagen.
Zhang Y, Zhang Y, Xie Y, Gao Y, Ma J, Yuan J, Li J, Wang J, Li L, Zhang J, Chu L. J Ethnopharmacol. 2013 Jul 9;148(2):671-81. Doi: 10.1016/J.Jep.2013.05.028. Epub 2013 May 22. Multitargeted Inhibition Of Hepatic Fibrosis In Chronic Iron-Overloaded...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Boster offers many ascites grade monoclonal antibodies that are sold at 100 microgram size. Does the 100 micrograms refer to amount of target-specific antibody? Keyword: concentration
A: MA1029 is lyophilized, and the lyophilized lump contain 100ug target-specific antibody and other protein as the agent for protein protection, the ratio is 1:20. The antibody is supplied as mouse ascites, it is not purified. We just detect it in WB.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to col3a1 antibody, type iii collagen antibody