Click image to see more details
Product Info Summary
| SKU: | A02844 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-Collagen VI alpha3 COL6A3 Antibody
SKU/Catalog Number
A02844
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen VI alpha3 COL6A3 Antibody (Catalog# A02844). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen VI alpha3 COL6A3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02844)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Collagen VI alpha3. Immunogen sequence location: 2261-2310
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02844 is reactive to COL6A3 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
343.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02844 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using Collagen VI alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen VI alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Collagen VI alpha3 COL6A3 Antibody (A02844)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Collagen VI alpha3 COL6A3 Antibody
Question
Will anti-Collagen VI alpha3 antibody A02844 work on bovine IHC with uterine cervix?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Our lab technicians have not validated anti-Collagen VI alpha3 antibody A02844 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Collagen VI alpha3 antibody A02844 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine uterine cervix in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
We are currently using anti-Collagen VI alpha3 antibody A02844 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-Collagen VI alpha3 antibody (A02844) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
Would A02844 anti-Collagen VI alpha3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
You can see on the product datasheet, A02844 anti-Collagen VI alpha3 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-08
Question
Here is the WB image, lot number and protocol we used for plasma using anti-Collagen VI alpha3 antibody A02844. Please let me know if you require anything else.
W. Anderson
Verified customer
Asked: 2019-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-17
Question
Is this A02844 anti-Collagen VI alpha3 antibody reactive to the isotypes of COL6A3?
Verified Customer
Verified customer
Asked: 2018-08-07
Answer
The immunogen of A02844 anti-Collagen VI alpha3 antibody is A peptide derived from human Collagen VI alpha3. Immunogen sequence location: 2261-2310. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-07
Question
I have a question about product A02844, anti-Collagen VI alpha3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Carter
Verified customer
Asked: 2018-05-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02844 anti-Collagen VI alpha3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-24
Question
I see that the anti-Collagen VI alpha3 antibody A02844 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-18
