Product Info Summary
SKU: | PB9002 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™
SKU/Catalog Number
PB9002
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ catalog # PB9002. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9002)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human COX1 recombinant protein (Position: E318-L599). Human COX1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat COX1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9002 is reactive to PTGS1 in Human, Mouse, Rat
Applications
PB9002 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
65-72 kDa
Calculated molecular weight
68.686kDa
Background of COX-1
Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat L6 whole cell lysates,
Lane 4: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 antigen affinity purified polyclonal antibody (Catalog # PB9002) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX1/Cyclooxygenase 1/PTGS1 at approximately 65-72 kDa. The expected band size for COX1/Cyclooxygenase 1/PTGS1 is at 69 kDa.
Click image to see more details
Figure 2. IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. Flow Cytometry analysis of HEL cells using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
Overlay histogram showing HEL cells stained with PB9002 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PTGS1 (Source: Uniprot.org, NCBI)
Gene Name
PTGS1
Full Name
Prostaglandin G/H synthase 1
Weight
68.686kDa
Superfamily
prostaglandin G/H synthase family
Alternative Names
COX1; COX-1; COX1PGG/HS; COX3; cyclooxygenase-1; EC 1.14.99.1; PCOX1; PGH synthase 1; PGHS1; PGHS-1PHS1; PHS 1; prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 1; PTGHS; PTGS1 PTGS1 COX1, COX3, PCOX1, PES-1, PGG/HS, PGHS-1, PGHS1, PHS1, PTGHS prostaglandin-endoperoxide synthase 1 prostaglandin G/H synthase 1|PGH synthase 1|cyclooxygenase-1|prostaglandin H2 synthase 1|prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGS1, check out the PTGS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ (PB9002)
Hello CJ!
PB9002 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties
Down-Regulation of Cyclooxygenase-2 is Involved in Ischemic Postconditioning Protection Against Renal Ischemia Reperfusion Injury in Rats
Dietary supplementation with Celecoxib to prevent the welfare problem of tibial dyschondroplasia in broiler chickens
Aspirin Inhibits Fractalkine Expression in Atherosclerotic Plaques and Reduces Atherosclerosis in ApoE Gene Knockout Mice
Combination of fasudil and celecoxib promotes the recovery of injured spinal cord in rats better than celecoxib or fasudil alone
Tolerance of neurite outgrowth to Rho kinase inhibitors decreased by cyclooxygenase-2 inhibitor
Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™
Question
Can PB9002 be used for mouse samples?
Verified customer
Asked: 2020-11-30
Answer
Our lab worked on mouse samples with the Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ (PB9002) and failed to observe specific target band.
Boster Scientific Support
Answered: 2020-11-30
Question
See attached the WB image, lot number and protocol we used for hippocampus trachea using anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-18
Question
We are currently using anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
Do you have a BSA free version of anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002 available?
L. Jha
Verified customer
Asked: 2019-05-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-09
Question
you antibody to test anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002 on human hippocampus trachea for research purposes, then I may be interested in using anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Jones
Verified customer
Asked: 2016-12-23
Answer
The products we sell, including anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus trachea using anti-COX1/Cyclooxygenase 1/PTGS1 antibody PB9002. Let me know if you need anything else.
W. Carter
Verified customer
Asked: 2016-01-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-14
Question
Is a blocking peptide available for product anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002)?
P. Edwards
Verified customer
Asked: 2013-07-04
Answer
We do provide the blocking peptide for product anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-04