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PTGS1 antibody (PB9002) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of HEL cells using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002).
Overlay histogram showing HEL cells stained with PB9002 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with PB9002 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband®
PTGS1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
PTGS1 antibody was positively detected by WB in:
human HepG2 whole cell, human A431 whole cell, rat L6 whole cell, mouse C2C12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTGS1 antibody was positively detected by IHC in:
human breast cancer tissue, human colonic adenoma tissue, human lung cancer tissue, mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PTGS1 antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband®
View all PTGS1 Antibodies
View all PTGS1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human COX1 recombinant protein (Position: E318-L599). Human COX1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat COX1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65-72 kDa
Calculated Molecular Weight
68.7 kDa
Background of PTGS1
Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.
Cite This Product
Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9002)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
92%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Dog
91%
High Homology
Mouse
90%
High Homology
Rat
89%
Moderate Homology
Zebrafish
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PTGS1 antibody
Immunohistochemistry (IHC) protocol for PTGS1 antibody
Flow Cytometry (FC) protocol for PTGS1 antibody
Publications
Application:
Species:
Oxyphenbutazone suppresses non-canonical inflammasome activation and protects against LPS-induced sepsis
Published: 2026-05-19
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 42150290
MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import
Published: 2024-10-09
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Rat
PMID: 39409161
Insights into the interaction of cyclooxygenase and lipoxygenase with natural compound 3,4′,5,7-Tetrahydroxyflavone based on multi-spectroscopic and metabolomics
Published: 2022-08-31
Journal: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Application: WB
Impact Factor: 4.83
Species: Rat
PMID: 36067623
Integrated metabolomics and mechanism to reveal the protective effect of kaempferol on pulmonary arterial hypertension
Published: 2022-02-15
Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Application: WB
Impact Factor: 3.57
Species: Rat
PMID: 35202944
Dietary supplementation with Celecoxib to prevent the welfare problem of tibial dyschondroplasia in broiler chickens
Published: 2021-05-27
Journal: Livestock Science
Application: IHC
Impact Factor: 1.94
Species: Chicken
Celecoxib aggravates atherogenesis and upregulates leukotrienes in ApoE−/− mice and lipopolysaccharide-stimulated RAW264.7 macrophages
Published: 2019-03-01
Journal: ATHEROSCLEROSIS
Application: WB
Impact Factor: 4.26
Species: Mouse
PMID: 30875493
Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties
Published: 2014-06-03
Journal: PLoS One
Application: WB
Impact Factor: 3.53
Species: Rat
PMID: 24892779
Aspirin Inhibits Fractalkine Expression in Atherosclerotic Plaques and Reduces Atherosclerosis in ApoE Gene Knockout Mice
Published: 2009-12-08
Journal: CARDIOVASCULAR DRUGS AND THERAPY
Application: IHC
Impact Factor: 2.45
Species: Mouse
PMID: 19997773
Down-Regulation of Cyclooxygenase-2 is Involved in Ischemic Postconditioning Protection Against Renal Ischemia Reperfusion Injury in Rats
Published: 2009-11-14
Journal: TRANSPLANTATION PROCEEDINGS
Application: WB
Impact Factor: 1.06
Species: Rat
PMID: 19917349
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Product Promise
We promise Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9002
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026