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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human MMP-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0458)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: F20-N469
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0458 is reactive to MMP1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0458 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-1
Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases. MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
|EK0458-CAP||Anti-Human MMP1 Pre-coated 96-well strip microplate||1|
|EK0458-ST||Human MMP1 Standard||2 vials, 10 ng/tube|
|EK0458-DA||Human MMP1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP1 (Source: Uniprot.Org, NCBI)
peptidase M10A family
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 22.214.171.124; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1 MMP1 CLG, CLGN matrix metallopeptidase 1 interstitial collagenase|fibroblast collagenase|matrix metallopeptidase 1 (interstitial collagenase)|matrix metalloprotease 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP1, check out the MMP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0458 has been cited in 20 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tsuji G,Takai-Yumine A,Kato T,Furue M.Metalloproteinase 1 downregulation in neurofibromatosis 1: Therapeutic potential of antimalarial hydroxychloroquine and chloroquine.Cell Death Dis.2021 May 19;12(6):513.doi:10.1038/s41419-021-03802-9.PMID:34011935;PMCID:PMC8134427.
EK0458 usage in article: APP:ELISA, SAMPLE:DERMAL FIBROBLAST CELL, DILUTION:NA
Marcheggiani F,Kordes S,Cirilli I,Orlando P,Silvestri S,Vogelsang A,Möller N,Blatt T,Weise JM,Damiani E,Tiano L.Anti-ageing effects of Ubiquinone and Ubiquinol in a senescence model of human dermal fibroblasts.Free Radic Biol Med.2021 Jan 19:S0891-5849(21
EK0458 usage in article: APP:ELISA, SAMPLE:HDF CELL, DILUTION:NA
A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction
Evaluation of inductive effects of different concentrations of cyclosporine A on MMP-1, MMP-2, MMP-3, TIMP-1, and TIMP-2 in fetal and adult human gingival fibroblasts
MMP, VEGF and TIMP as prognostic factors in recurring bladder cancer
Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma
MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway
Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging
Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts
Man S, Gao W, Zhang Y, Liu Z, Yan L, Huang L, Liu C. Cancer Biol Ther. 2011 Mar 15;11(6):592-8. Epub 2011 Mar 15. Formosanin C-Inhibited Pulmonary Metastasis Through Repression Of Matrix Metalloproteinases On Mouse Lung Adenocarcinoma.
Deng Cl, Ling St, Liu Xq, Zhao Yj, Lv Yf. Exp Ther Med. 2015 Mar;9(3):992-998. Epub 2015 Jan 20. Decreased Expression Of Matrix Metalloproteinase-1 In The Maternal Umbilical Serum, Trophoblasts And Decidua Leads To Preeclampsia.
Nikniaz Z, Ostadrahimi A, Mahdavi R, Ebrahimi Aa, Nikniaz L. Complement Ther Med. 2014 Oct;22(5):864-9. Doi: 10.1016/J.Ctim.2014.07.004. Epub 2014 Jul 16. Effects Of Elaeagnus Angustifolia L. Supplementation On Serum Levels Of Inflammatory Cytokin...
Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...
??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...
Hopps E, Lo Presti R, Montana M, Noto D, Averna Mr, Caimi G. J Investig Med. 2013 Aug;61(6):978-83. Doi: 10.231/Jim.0B013E318294E9Da. Gelatinases And Their Tissue Inhibitors In A Group Of Subjects With Metabolic Syndrome.
Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...
Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.
Huang J, Luo X, Lu J, Chen J, Zuo C, Xiang Y, Yang S, Tan L, Kang J, Bi Z. Lasers Med Sci. 2011 May;26(3):381-7. Doi: 10.1007/S10103-010-0870-1. Epub 2010 Dec 16. Ipl Irradiation Rejuvenates Skin Collagen Via The Bidirectional Regulation Of Mmp-1 ...
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...
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