Anti-MED15 Antibody Picoband™ (monoclonal, 6F4)

Boster Bio Anti-MED15 Antibody Picoband™ (monoclonal, 6F4) catalog # M03568. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M03568
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-MED15 Antibody Picoband™ (monoclonal, 6F4)

See all MED15 products

SKU/Catalog Number

M03568

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MED15 Antibody Picoband™ (monoclonal, 6F4) catalog # M03568. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED15 Antibody Picoband™ (monoclonal, 6F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03568)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

6F4

Isotype

IgG1

Immunogen

E. coli-derived human MED15 recombinant protein (Position: M1-A285).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M03568 is reactive to MED15 in Human

Applications

M03568 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For MED15 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96RN5

Gene ID

51586

Gene Name

MED15

Full Name

Mediator of RNA polymerase II transcription subunit 15

Weight

86.753kDa

Superfamily

Mediator complex subunit 15 family

Alternative Names

ARC105Arc105; CAG7A; CTG repeat protein 7a; CTG7A; FLJ42282; FLJ42935; mediator complex subunit 15PC2 glutamine/Q-rich-associated protein; mediator of RNA polymerase II transcription subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein; PC2-glutamine-rich-associated protein; PCQAPDKFZp686A2214; Positive cofactor 2 glutamine/Q-rich-associated protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1DKFZp762B1216; TIG-1TPA-inducible gene 1 protein; TNRC7; TPA inducible gene-1; TPA inducible protein; trinucleotide repeat containing 7,105-kD; MED15 ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7 mediator complex subunit 15 mediator of RNA polymerase II transcription subunit 15|CTG repeat protein 7a|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein|PC2 glutamine/Q-rich-associated protein|PC2-glutamine-rich-associated protein|TPA inducible gene-1|TPA inducible protein|TPA-inducible gene 1 protein|activator-recruited cofactor 105 kDa component|activator-recruited cofactor, 105-kD|positive cofactor 2, glutamine/Q-rich-associated protein|trinucleotide repeat containing 7|trinucleotide repeat-containing gene 7 protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MED15, check out the MED15 Infographic

MED15 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MED15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-MED15 Antibody Picoband™ (monoclonal, 6F4)

Question

I was wanting to use your anti-MED15 antibody (monoclonal, 6F4) for IF for human megakaryocyte placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human megakaryocyte placenta identification?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

It shows on the product datasheet, M03568 anti-MED15 antibody (monoclonal, 6F4) has been validated for Flow Cytometry, IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human megakaryocyte placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-23

Question

Is this M03568 anti-MED15 antibody (monoclonal, 6F4) reactive to the isotypes of MED15?

Verified Customer

Verified customer

Asked: 2018-07-30

Answer

The immunogen of M03568 anti-MED15 antibody (monoclonal, 6F4) is E. coli-derived human MED15 recombinant protein (Position: M1-A285). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-07-30

Question

We want to test anti-MED15 antibody (monoclonal, 6F4) M03568 on human megakaryocyte placenta for research purposes, then I may be interested in using anti-MED15 antibody (monoclonal, 6F4) M03568 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-20

Answer

The products we sell, including anti-MED15 antibody (monoclonal, 6F4) M03568, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-20

Question

We are currently using anti-MED15 antibody (monoclonal, 6F4) M03568 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

M. Mangal

Verified customer

Asked: 2015-03-23

Answer

The anti-MED15 antibody (monoclonal, 6F4) (M03568) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-03-23

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M03568
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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