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Product Info Summary
| SKU: | M03568 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MED15 Antibody Picoband® (monoclonal, 6F4)
SKU/Catalog Number
M03568
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED15 Antibody Picoband® (monoclonal, 6F4) catalog # M03568. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED15 Antibody Picoband® (monoclonal, 6F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03568)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6F4
Isotype
Mouse IgG1
Immunogen
E. coli-derived human MED15 recombinant protein (Position: M1-A285).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03568 is reactive to MED15 in Human
Observed Molecular Weight
86 kDa
Calculated molecular weight
86.8 kDa
Background of MED15
Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03568 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
WB: human K562 whole cell, human U87 whole cell, human CACO-2 whole cell, human HL-60 whole cell, human HepG2 whole cell, human Hela whole cell, human PC-3 whole cell
ICC/IF: A431 cell
FCM: U20S cell
Validation Images & Assay Conditions
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Western blot analysis of MED15 using anti-MED15 antibody (M03568).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MED15 antigen affinity purified monoclonal antibody (Catalog # M03568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MED15 at approximately 86KD. The expected band size for MED15 is at 86KD.
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IF analysis of MED15 using anti-MED15 antibody (M03568).
MED15 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-MED15 Antibody (M03568) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of U20S cells using anti- MED15 antibody (M03568).
Overlay histogram showing U20S cells stained with M03568 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MED15 Antibody (M03568, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-MED15 Antibody Picoband® (monoclonal, 6F4) (M03568)
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4 Customer Q&As for Anti-MED15 Antibody Picoband® (monoclonal, 6F4)
Question
I was wanting to use your anti-MED15 antibody (monoclonal, 6F4) for IF for human megakaryocyte placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human megakaryocyte placenta identification?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
It shows on the product datasheet, M03568 anti-MED15 antibody (monoclonal, 6F4) has been validated for Flow Cytometry, IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human megakaryocyte placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
Is this M03568 anti-MED15 antibody (monoclonal, 6F4) reactive to the isotypes of MED15?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
The immunogen of M03568 anti-MED15 antibody (monoclonal, 6F4) is E. coli-derived human MED15 recombinant protein (Position: M1-A285). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-30
Question
We want to test anti-MED15 antibody (monoclonal, 6F4) M03568 on human megakaryocyte placenta for research purposes, then I may be interested in using anti-MED15 antibody (monoclonal, 6F4) M03568 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-20
Answer
The products we sell, including anti-MED15 antibody (monoclonal, 6F4) M03568, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-20
Question
We are currently using anti-MED15 antibody (monoclonal, 6F4) M03568 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
M. Mangal
Verified customer
Asked: 2015-03-23
Answer
The anti-MED15 antibody (monoclonal, 6F4) (M03568) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-23
