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Pack Size:100μg/vial
Validated Species:Human, Mouse, Rat
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Product Name Anti-Cpn10 Antibody
SKU/Catalog Number PA1790
Description Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial(HSPE1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-Cpn10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1790)
Replacement Item This antibody may replace the following items: sc-102429|sc-102428 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name HSPE1
Protein Name 10 kDa heat shock protein, mitochondrial(Hsp10)
Molecular Weight 10932 MW
Protein Function Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Subcellular Localization Mitochondrion matrix.
Uniprot ID P61604
Alternative Names 10 kDa heat shock protein, mitochondrial;Hsp10;10 kDa chaperonin;Chaperonin 10;CPN10;Early-pregnancy factor;EPF;HSPE1;
Research Areas |signal transduction|protein trafficking|chaperones|heat shock proteins| signal transduction|metabolism|mitochondrial| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|types of disease|cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for 10 kDa heat shock protein, mitochondrial(Hsp10)

HSPE1(heat shock 10kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

Anti-Cpn10 Antibody Images

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Anti-Cpn10 Antibody
Anti-Cpn10 antibody, PA1790, ICC
ICC: A549 Cell
Anti-Cpn10 Antibody
Anti-Cpn10 antibody, PA1790, Western blotting
All lanes: Anti Cpn10 (PA1790) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Testis Tissue Lysate at 50ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: MM231 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
Anti-Cpn10 Antibody
Anti-Cpn10 antibody, PA1790, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Anti-Cpn10 Antibody
Anti-Cpn10 antibody, PA1790, IHC(P)
IHC(P): Mouse Intestine Tissue
Anti-Cpn10 Antibody
Anti-Cpn10 antibody, PA1790, IHC(P)
IHC(P): Rat Intestine Tissue
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Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to groes antibody, hsp10 antibody, cpn10 antibody