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Product Info Summary
| SKU: | PA1802 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Heat shock protein beta-2 HSPB2 Antibody Picoband®
SKU/Catalog Number
PA1802
BA2971 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heat shock protein beta-2 HSPB2 Antibody catalog # PA1802. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heat shock protein beta-2 HSPB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1802)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HSPB2, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1802 is reactive to HSPB2 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
20.2 kDa
Background of HSPB2
Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1802 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Positive Control
WB: human U20S whole cell, human PC-3 whole cell, rat H9C2 whole cell, rat heart tissue, mouse C2C12 whole cell, mouse heart tissue
IHC-F: Rat Cardiac Muscle Tissue
ICC: HELA Cell
Validation Images & Assay Conditions
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Western blot analysis of HSPB2 using anti-HSPB2 antibody (PA1802).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: rat H9C2 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: mouse C2C12 whole cell lysates,
Lane 6: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PA1802) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 23 kDa. The expected band size for HSPB2 is at 20 kDa.
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IHC analysis of HSPB2 using anti-HSPB2 antibody (PA1802).
HSPB2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPB2 Antibody (PA1802) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-HSPB2 antibody, PA1802, IHC(F)
IHC(F): Rat Cardiac Muscle Tissue
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Anti-HSPB2 antibody, PA1802, ICC
ICC: HELA Cell
Specific Publications For Anti-Heat shock protein beta-2 HSPB2 Antibody Picoband® (PA1802)
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3 Customer Q&As for Anti-Heat shock protein beta-2 HSPB2 Antibody Picoband®
Question
I was wanting to use your anti-HSPB2 antibody for IHC for rat muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat muscle identification?
B. Krishna
Verified customer
Asked: 2019-06-25
Answer
It shows on the product datasheet, PA1802 anti-HSPB2 antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
My question regarding product PA1802, anti-HSPB2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Miller
Verified customer
Asked: 2015-03-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1802 anti-HSPB2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-10
Question
Will PA1802 anti-HSPB2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Brown
Verified customer
Asked: 2015-02-17
Answer
You can see on the product datasheet, PA1802 anti-HSPB2 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-17
