Anti-Phospho-CREB S133 CREB1 Antibody

Product Name

Anti-Phospho-CREB S133 CREB1 Antibody

View all CREB1 Antibodies

SKU/Catalog Number

P00577

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-CREB S133 CREB1 Antibody (Catalog # P00577). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-CREB S133 CREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00577)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactive Species

P00577 is reactive to CREB1 in Human, Mouse, Rat

Observed Molecular Weight

52 kDa

Calculated molecular weight

36.7 kDa

Background of CREB1

The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P00577 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,000 - 1:6,000
IHC: 20 µg/ml
WB: 1:500 - 1:2,000
Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide.  This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates.  Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots.  Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB.  No cross-reactivity occurs with non-phosphorylated CREB.  For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining.  Although not tested this antibody is likely functional by FACS and immunoprecipitation.

Validation Images & Assay Conditions

See full target information CREB1

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