Anti-NMDAR1/GRIN1 Antibody

Boster Bio Anti-NMDAR1/GRIN1 Antibody catalog # PA1222. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 3 publication(s).

Product Info Summary

SKU: PA1222
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-NMDAR1/GRIN1 Antibody

See all GRIN1 products

SKU/Catalog Number

PA1222

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NMDAR1/GRIN1 Antibody catalog # PA1222. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NMDAR1/GRIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1222)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1 (36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1222 is reactive to GRIN1 in Human, Mouse, Rat

Applications

PA1222 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For GRIN1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q05586

Gene ID

2902

Gene Name

GRIN1

Full Name

Glutamate receptor ionotropic, NMDA 1

Weight

105.373kDa

Superfamily

glutamate-gated ion channel (TC 1.A.10.1) family

Alternative Names

glutamate [NMDA] receptor subunit zeta-1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA1; NMDAR1glutamate [NMDA] receptor subunit zeta 1; NMD-R1; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1 GRIN1 GluN1, MRD8, NDHMSD, NDHMSR, NMD-R1, NMDA1, NMDAR1, NR1 glutamate ionotropic receptor NMDA type subunit 1 glutamate receptor ionotropic, NMDA 1|N-methyl-D-aspartate receptor channel, subunit zeta-1|N-methyl-D-aspartate receptor subunit NR1|glutamate [NMDA] receptor subunit zeta-1|glutamate receptor, ionotropic, N-methyl D-aspartate 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GRIN1, check out the GRIN1 Infographic

GRIN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GRIN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1222 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation

Wang Xy, Wang Hw, Fu Xh, Zhang Wq, Wu Xy, Guo Qy, Wang Xm. Eur J Paediatr Neurol. 2012 Sep;16(5):492-500. Doi: 10.1016/J.Ejpn.2012.01.005. Epub 2012 Jan 20. Expression Of N-Methyl-D-Aspartate Receptor 1 And Its Phosphorylated State In Basal Gangli...

Shang Lh, Luo Zq, Deng Xd, Wang Mj, Huang Fr, Feng Dd, Yue Sj. Nitric Oxide. 2010 Dec 15;23(4):327-31. Doi: 10.1016/J.Niox.2010.09.004. Epub 2010 Sep 25. Expression Of N-Methyl-D-Aspartate Receptor And Its Effect On Nitric Oxide Production Of Rat ...

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17 Customer Q&As for Anti-NMDAR1/GRIN1 Antibody

Question

See attached the WB image, lot number and protocol we used for cerebellum hippocampus using anti-NMDAR1/GRIN1 antibody PA1222. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-02

Question

I was wanting to use your anti-NMDAR1/GRIN1 antibody for WB for human cerebellum hippocampus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cerebellum hippocampus identification?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

As indicated on the product datasheet, PA1222 anti-NMDAR1/GRIN1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum hippocampus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-02

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum hippocampus using anti-NMDAR1/GRIN1 antibody PA1222. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-16

Question

We have been able to see staining in rat cerebellum hippocampus. Do you have any suggestions? Is anti-NMDAR1/GRIN1 antibody supposed to stain cerebellum hippocampus positively?

V. Carter

Verified customer

Asked: 2019-11-12

Answer

Based on literature cerebellum hippocampus does express GRIN1. Based on Uniprot.org, GRIN1 is expressed in anterior cingulate cortex, brain, cerebellum hippocampus, among other tissues. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821

Boster Scientific Support

Answered: 2019-11-12

Question

I see that the anti-NMDAR1/GRIN1 antibody PA1222 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-08

Question

Do you have a BSA free version of anti-NMDAR1/GRIN1 antibody PA1222 available?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NMDAR1/GRIN1 antibody PA1222 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-02

Question

Would PA1222 anti-NMDAR1/GRIN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

You can see on the product datasheet, PA1222 anti-NMDAR1/GRIN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-19

Question

My colleagues were content with the WB result of your anti-NMDAR1/GRIN1 antibody. However we have seen positive staining in cerebellum hippocampus cell membrane using this antibody. Is that expected? Could you tell me where is GRIN1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

Based on literature, cerebellum hippocampus does express GRIN1. Generally GRIN1 expresses in cell membrane. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821

Boster Scientific Support

Answered: 2019-08-26

Question

I have a question about product PA1222, anti-NMDAR1/GRIN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1222 anti-NMDAR1/GRIN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-14

Question

We are currently using anti-NMDAR1/GRIN1 antibody PA1222 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-26

Answer

The anti-NMDAR1/GRIN1 antibody (PA1222) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-26

Question

Is this PA1222 anti-NMDAR1/GRIN1 antibody reactive to the isotypes of GRIN1?

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

The immunogen of PA1222 anti-NMDAR1/GRIN1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1(36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-26

Question

Is a blocking peptide available for product anti-NMDAR1/GRIN1 antibody (PA1222)?

T. Jones

Verified customer

Asked: 2018-02-13

Answer

We do provide the blocking peptide for product anti-NMDAR1/GRIN1 antibody (PA1222). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-13

Question

We ordered your anti-NMDAR1/GRIN1 antibody for IHC on brain in a previous experiment. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining brain as well as cerebellum hippocampus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-02-12

Answer

I looked at the website and datasheets of our anti-NMDAR1/GRIN1 antibody and I see that PA1222 has been tested on human in both IHC and WB. Thus PA1222 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-02-12

Question

I am interested in using your anti-NMDAR1/GRIN1 antibody for negative regulation of nmda receptor-mediated neuronal transmission studies. Has this antibody been tested with western blotting on mm453 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-11-24

Answer

We appreciate your inquiry. This PA1222 anti-NMDAR1/GRIN1 antibody is validated on rat heart tissue, brain tissue, tissue lysate, liver tissue, mm453 cell lysate, mm231 cell lysate, hela cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-11-24

Question

Will anti-NMDAR1/GRIN1 antibody PA1222 work for WB with cerebellum hippocampus?

M. Anderson

Verified customer

Asked: 2016-07-12

Answer

According to the expression profile of cerebellum hippocampus, GRIN1 is highly expressed in cerebellum hippocampus. So, it is likely that anti-NMDAR1/GRIN1 antibody PA1222 will work for WB with cerebellum hippocampus.

Boster Scientific Support

Answered: 2016-07-12

Question

I am interested in to test anti-NMDAR1/GRIN1 antibody PA1222 on human cerebellum hippocampus for research purposes, then I may be interested in using anti-NMDAR1/GRIN1 antibody PA1222 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Dhar

Verified customer

Asked: 2015-12-28

Answer

The products we sell, including anti-NMDAR1/GRIN1 antibody PA1222, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-12-28

Question

Does anti-NMDAR1/GRIN1 antibody PA1222 work on primate IHC with cerebellum hippocampus?

C. Moore

Verified customer

Asked: 2015-03-19

Answer

Our lab technicians have not validated anti-NMDAR1/GRIN1 antibody PA1222 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NMDAR1/GRIN1 antibody PA1222 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cerebellum hippocampus in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-03-19

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