Rabbit IgG polyclonal antibody for Glutamate receptor ionotropic, NMDA 1(GRIN1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-NMDAR1/GRIN1 Antibody
See all GRIN1 primary antibodies, ELISA kits and proteins
|Storage & Handling||For proper storage of Anti-NMDAR1/GRIN1 Antibody: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for NMDAR1/GRIN1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NMDAR1/GRIN1 information: Molecular Weight: 105373 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Enriched in postsynaptic plasma membrane and postsynaptic densities.|
|Cite This Product||Anti-NMDAR1/GRIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1222)|
|Specificity||Anti-NMDAR1/GRIN1 Antibody (PA1222) reacts with Human, Mouse, Rat GRIN1, in native form and recombinant. Superfamily members of GRIN1 are not reactive to PA1222.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1(36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-NMDAR1/GRIN1 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-NMDAR1/GRIN1 Antibody (PA1222).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-NMDAR1 antibody, PA1222, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Heart Tissue Lysate
Lane 5: MM453 Cell Lysate
Lane 6: MM231 Cell Lysate
Lane 7: HELA Cell Lysate
Anti-NMDAR1 antibody, PA1222, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Glutamate receptor ionotropic, NMDA 1|
|Alternative Names||Glutamate receptor ionotropic, NMDA 1;GluN1;Glutamate [NMDA] receptor subunit zeta-1;N-methyl-D-aspartate receptor subunit NR1;NMD-R1;GRIN1;NMDAR1;|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Enriched in postsynaptic plasma membrane and postsynaptic densities. .|
|Molecular Weight||105373 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity). .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||Glutamate [NMDA] receptor subunit zeta-1 is a protein that in humans is encoded by the GRIN1 gene. The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.|
Other Recommended Resources
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Publishing with Anti-NMDAR1/GRIN1 Antibody (PA1222)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to nmdar antibody, nr1 antibody, nmdar1 antibody