Rat BDNF PicoKine® Quick ELISA Kit

Bdnf ELISA kit, 90-minute one-step protocol

Rat BDNF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Product Info Summary

SKU: FEK0308
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate)
Assay Time: 90 Minutes

Product Name

Rat BDNF PicoKine® Quick ELISA Kit

See all Bdnf products

SKU/Catalog Number

FEK0308

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat BDNF PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat BDNF PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0308)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: H129-R247

Sensitivity

<15 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0308 is reactive to Bdnf in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK0308 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Bdnf

Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

Kit Components

Catalog numberDescription QuantityVolume
FEK0308-CAPAnti-Rat Bdnf Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0308-STRat Bdnf Standard 2 10 ng/tube
FEK0308-HHRP Linked Anti-Rat Bdnf Antibody 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0140.0390.0760.1200.2550.4851.0201.955

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)95302947882891024
Standard deviation4.9416.9172.914.7521.6786.01
CV (%)5.2%5.6%7.7%5.4%7.5%8.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Bdnf in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1959894105986.176.3%
Sample 230228626529628712.344.3%
Sample 394598391496295146.594.9%
*number of samples for each test n=16.

Gene/Protein Information For Bdnf (Source: Uniprot.Org, NCBI)

Uniprot ID

P23363

Gene ID

24225

Gene Name

Bdnf

Full Name

Brain-derived neurotrophic factor

Weight

28.109kDa

Superfamily

NGF-beta family

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin Bdnf|brain-derived neurotrophic factor|brain-derived neurotrophic factor|brain derived neurothrophic factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bdnf, check out the Bdnf Infographic

Bdnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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