Anti-CRP/C Reactive Protein Antibody

Boster Bio Anti-CRP/C Reactive Protein Antibody catalog # A00249. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: A00249
Size: 100μl
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CRP/C Reactive Protein Antibody

See all C-Reactive Protein/CRP products

SKU/Catalog Number

A00249

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CRP/C Reactive Protein Antibody catalog # A00249. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CRP/C Reactive Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00249)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CRP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00249 is reactive to CRP in Human, Rat

Applications

A00249 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC-P, 1:100-300

Validation Images & Assay Conditions

Gene/Protein Information For CRP (Source: Uniprot.Org, NCBI)

Uniprot ID

P02741

Gene ID

1401

Gene Name

CRP

Full Name

C-reactive protein

Weight

25.039kDa

Superfamily

pentraxin family

Alternative Names

C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CRP, check out the CRP Infographic

CRP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CRP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00249

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16 Customer Q&As for Anti-CRP/C Reactive Protein Antibody

Question

Is a blocking peptide available for product anti-CRP/C Reactive Protein antibody (A00249)?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

We do provide the blocking peptide for product anti-CRP/C Reactive Protein antibody (A00249). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-05-06

Question

We purchased anti-CRP/C Reactive Protein antibody for WB on liver a few months ago. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining liver as well as urinary bladder in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

I took a look at the website and datasheets of our anti-CRP/C Reactive Protein antibody and it seems that A00249 has been tested on human in both WB and IHC. Thus A00249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-17

Question

I have a question about product A00249, anti-CRP/C Reactive Protein antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00249 anti-CRP/C Reactive Protein antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-26

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-CRP/C Reactive Protein antibody A00249. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-06

Question

I was wanting to use your anti-CRP/C Reactive Protein antibody for WB for rat right lobe of liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat right lobe of liver identification?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

You can see on the product datasheet, A00249 anti-CRP/C Reactive Protein antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right lobe of liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-29

Question

Our team were happy with the WB result of your anti-CRP/C Reactive Protein antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

From literature, liver does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-28

Question

Would anti-CRP/C Reactive Protein antibody A00249 work for WB with right lobe of liver?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

According to the expression profile of right lobe of liver, CRP is highly expressed in right lobe of liver. So, it is likely that anti-CRP/C Reactive Protein antibody A00249 will work for WB with right lobe of liver.

Boster Scientific Support

Answered: 2019-11-20

Question

We are currently using anti-CRP/C Reactive Protein antibody A00249 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

The anti-CRP/C Reactive Protein antibody (A00249) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-07

Question

you antibody using your anti-CRP/C Reactive Protein antibody for inflammatory response studies. Has this antibody been tested with western blotting on liver cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

Thanks for your inquiry. This A00249 anti-CRP/C Reactive Protein antibody is tested on mouse liver, liver cells. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-24

Question

Do you have a BSA free version of anti-CRP/C Reactive Protein antibody A00249 available?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CRP/C Reactive Protein antibody A00249 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-21

Question

Would A00249 anti-CRP/C Reactive Protein antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

You can see on the product datasheet, A00249 anti-CRP/C Reactive Protein antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-05-29

Question

We have observed staining in rat liver. Are there any suggestions? Is anti-CRP/C Reactive Protein antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2018-03-23

Answer

From what I have seen in literature liver does express CRP. From what I have seen in Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-03-23

Question

Please see the WB image, lot number and protocol we used for right lobe of liver using anti-CRP/C Reactive Protein antibody A00249. Please let me know if you require anything else.

J. Zhang

Verified customer

Asked: 2018-01-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-30

Question

Is this A00249 anti-CRP/C Reactive Protein antibody reactive to the isotypes of CRP?

N. Moore

Verified customer

Asked: 2017-10-12

Answer

The immunogen of A00249 anti-CRP/C Reactive Protein antibody is Synthesized peptide derived from the Internal region of human CRP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-12

Question

I see that the anti-CRP/C Reactive Protein antibody A00249 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-07-26

Question

I am interested in to test anti-CRP/C Reactive Protein antibody A00249 on rat right lobe of liver for research purposes, then I may be interested in using anti-CRP/C Reactive Protein antibody A00249 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Krishna

Verified customer

Asked: 2015-03-23

Answer

The products we sell, including anti-CRP/C Reactive Protein antibody A00249, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-03-23

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00249
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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