Mouse uPAR / uPA Receptor ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse uPAR. 96wells/kit, with removable strips.
Mouse uPAR / uPA Receptor ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse uPAR / uPA Receptor ELISA Kit PicoKine™
See all uPAR primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Upar/PLAUR with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Upar/PLAUR tissue specificity: Expressed in angiogenic endothelial cells (at protein level). .|
|Cite This Product||Mouse uPAR / uPA Receptor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1215)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: L24-T297|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Plaur (Source: Uniprot.org, NCBI)
|NCBI Gene Id||18793|
|Species Of This Entry||Mouse|
|Protein Name||Urokinase plasminogen activator surface receptor|
|Alternative Names||uPAR|CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Glycosylation; Gpi Anchoring; Biotinylation; Dephosphorylation; Iodination; Deglycosylation; Phosphorothioation; Nitration; Reduction; Acetylation; Amidation; Prenylation; Farnesylation; Methylation; Oxidation|
|Gene Location||7|7 A3, on Chromasome 7, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Plaur (Source: Uniprot.org, NCBI)
|Protein Function||Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Cell Invasion; Neoplasm Metastasis; Tissue Adhesions; Carcinoma; Neoplasm Invasiveness; Mammary Neoplasms; Malignant Paraganglionic Neoplasm; Malignant Neoplasm Of Breast; Tumor Angiogenesis; Inflammation; Pathologic Neovascularization; Tumor Progression; Lung Neoplasms; Adenocarcinoma; Malignant Tumor Of Colon; Prostatic Neoplasms; Malignant Neoplasm Of Prostate|
|Related Pathways||Proteolysis; Plasminogen Activation; Cell Migration; Angiogenesis; Cell Adhesion; Localization; Cell Proliferation; Secretion; Fibrinolysis; Pathogenesis; Chemotaxis; Cell Motility; Tissue Remodeling; Wound Healing; Endocytosis; Cell Growth; Regeneration; Tube Formation; Cell Differentiation; Inflammatory Response|
|Background||The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1|
|Scientific References||PMID: 1661735 by Kristensen P., et al. Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract.|
PMID: 7929309 by Suh T.T., et al. The murine urokinase-type plasminogen activator receptor gene.
Kit Components And Assay QC Data Details Of Mouse uPAR / uPA Receptor ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1215-CAP||96-well plate precoated with anti-Mouse uPAR antibody||1|
|EK1215-ST||lyophilized recombinant Mouse uPAR standard||10ng/tube|
|EK1215-DA||biotinylated anti-Mouse uPAR antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1215 Mouse uPAR / uPA Receptor ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse uPAR / uPA Receptor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse uPAR / uPA Receptor ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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