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Product Info Summary
| SKU: | M00020 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CTLA4 (CD152) Monoclonal Antibody
SKU/Catalog Number
M00020
BM5388 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CTLA4 (CD152) Monoclonal Antibody catalog # M00020. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTLA4 (CD152) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00020)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADCD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CTLA4 (CD152)
Reactive Species
M00020 is reactive to CTLA4 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
24.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00020 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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HPM treatment restored body weight and increased spleen index in immunosuppressed rats. A Schematic diagram of the acupoints used in this experiment. B Dimensional specifications of the HPM in the experiment. C Schematic showing timeline for CTX induction, HPM treatment and behaviour experiments. D , E Mean weight of each group (n = 8 per each group). Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.001, *** p < 0.001 vs NG; # p < 0.05, ### p < 0.001 vs CTX. SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Immunosuppressed rats exhibit depressive/anxiety-like behavior, which is attenuated after HPM binding inhibitor treatment. A , B Schematic representation of OFT (n = 8 per each group). C , D Schematic representation of EPM (n = 5 per each group). Data were analyzed using Kruskal–Wallis H- statistic test. * p < 0.05, ** p < 0.01vs NG. NS: no significance; OFT: open-field test; EPM: elevated plus-maze; NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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The number of WBCs was significantly reduced in rats after cyclophosphamide injection and increased after HPM treatment. A WBCs and their subtype percentage results. (n = 3–8 per each group). B – D RBC, hemoglobin and platelet counts (n = 8 per each group). Data were analyzed using One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.01, *** p < 0.001vs NG; # p < 0.05 vs CTX; % p < 0.05 vs HPM. ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NS: no significance; NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Histological analysis of spleen in immunosuppressed rats (H&E staining, bar = 100 μm). CV, central vein; LN, lymph nodule; MZ, marginal zone; RP, red pulp, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Levels of CD28 ( A ), CTLA-4 ( B ), B7-1 ( C ), B7-2 ( D ), α-MSH ( E ), TrkB ( F ), 5-HT ( G ), BDNF ( H ) in serum of immunosuppressed rats (n = 8 per each group). Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; # p < 0.05, ## p < 0.01, ### p < 0.001 vs CTX; %% p < 0.01, %%% p < 0.001 vs HPM. α-MSH: α-Melanocyte-stimulating hormone; TrkB: Tropomyosin receptor kinase B; 5-HT: 5-hydroxytryptamine; BDNF: Brain-derived neurotrophic factor; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Effect of HPM treatment on T lymphocytes in the spleens of immunosuppressed rats. A CD4+, CD8+ cells were gated by the protocol. B Flow cytometric identification of CD4 + , CD8 + cells in the spleen. C , D Absolute count statistics of CD4+, CD8+ cells between different groups in spleen tissue (n = 5 per each group). Data were analyzed using Kruskal–Wallis H-statistic test. ** p < 0.01, *** p < 0.001vs NG; # p < 0.05 vs CTX. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Results of the CD28 mRNA level and correlation between CD28 and B7-1 in spleen of immunosuppressed rats (n = 3–5 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CD28 IOD results; C CD28 mRNA level; D CD28 and B7-1 colocalization results. Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed and Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; ## p < 0.01, ### p < 0.001 vs CTX; % p < 0.05, %%% p < 0.001 vs HPM. IOD: integrated optical density; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Results of the correlation between CD28 and B7-2 in spleen of immunosuppressed rats (n = 3 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CD28 and B7-2 colocalization results. Data are expressed as Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. ** p < 0.01 vs NG; ## p < 0.01 vs CTX. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Results of CTLA-4 mRNA level and the correlation between CTLA-4 and B7-1 in spleen of immunosuppressed rats (n = 3–5 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CTLA-4 IOD results; C CTLA-4 mRNA level; D B7-1 mRNA level; E CTLA- and B7-1 colocalization results. Data are expressed as means ± SD, One-way ANOVA tests with LSD-t post-hoc tests executed and Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs NG; ## p < 0.01, ### p < 0.001 vs CTX; %%% p < 0.001 vs HPM. IOD: integrated optical density; SD: standard deviation; ANOVA: analysis of variance; LSD-t: Least Significant Difference-t, NG: normal group; CTX: cyclophosphamide; HPM: Herbpartitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Results of the correlation between CTLA-4 and B7-2 in spleen of immunosuppressed rats (n = 3 per each group). A immunofluorescence results, a – f Pearson correlation scatterplot; B CTLA-4 and B7-2 colocalization results. Data are expressed as Pearson correlation coefficient, Kruskal–Wallis one-way ANOVA test. ## p < 0.01 vs CTX; %% p < 0.01 vs HPM. NG: normal group; CTX: cyclophosphamide; HPM: Herb-partitioned moxibustion; aCD28: anti-CD28; aCTLA-4: anti-CTLA-4; LEV: Levamisole
Index in PubMed under a CC BY license. PMID: 38369521
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Western blot analysis of CTLA4 (CD152) expression in NIH/3T3 cell lysate.
Specific Publications For Anti-CTLA4 (CD152) Monoclonal Antibody (M00020)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CTLA4 (CD152) Monoclonal Antibody
Question
Would M00020 anti-CTLA4 (CD152) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
It shows on the product datasheet, M00020 anti-CTLA4 (CD152) Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-13
Question
Is there a BSA free version of anti-CTLA4 (CD152) Monoclonal antibody M00020 available?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CTLA4 (CD152) Monoclonal antibody M00020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-CTLA4 (CD152) Monoclonal antibody M00020 work for ICC with lymphocyte?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to the expression profile of lymphocyte, CTLA4 is highly expressed in lymphocyte. So, it is likely that anti-CTLA4 (CD152) Monoclonal antibody M00020 will work for ICC with lymphocyte.
Boster Scientific Support
Answered: 2019-08-01
Question
I have a question about product M00020, anti-CTLA4 (CD152) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00020 anti-CTLA4 (CD152) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-CTLA4 (CD152) Monoclonal antibody M00020 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The anti-CTLA4 (CD152) Monoclonal antibody (M00020) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-22
Question
We purchased anti-CTLA4 (CD152) Monoclonal antibody for ICC on lymph node in a previous project. I am using human, and I plan to use the antibody for IF next. We need examining lymph node as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
I have checked the website and datasheets of our anti-CTLA4 (CD152) Monoclonal antibody and it seems that M00020 has been validated on human in both ICC and IF. Thus M00020 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-10
Question
I have attached the WB image, lot number and protocol we used for lymphocyte using anti-CTLA4 (CD152) Monoclonal antibody M00020. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
I see that the anti-CTLA4 (CD152) Monoclonal antibody M00020 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-30
Question
Is this M00020 anti-CTLA4 (CD152) Monoclonal antibody reactive to the isotypes of CTLA4?
J. Taylor
Verified customer
Asked: 2019-02-26
Answer
The immunogen of M00020 anti-CTLA4 (CD152) Monoclonal antibody is A synthesized peptide derived from human CTLA4 (CD152). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-26
Question
I was wanting to use your anti-CTLA4 (CD152) Monoclonal antibody for ICC for human lymphocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymphocyte identification?
P. Krishna
Verified customer
Asked: 2019-02-12
Answer
As indicated on the product datasheet, M00020 anti-CTLA4 (CD152) Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-CTLA4 (CD152) Monoclonal antibody M00020. Let me know if you need anything else.
J. Patel
Verified customer
Asked: 2018-12-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-28
Question
We have seen staining in rat lymph node. Are there any suggestions? Is anti-CTLA4 (CD152) Monoclonal antibody supposed to stain lymph node positively?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
According to literature lymph node does express CTLA4. According to Uniprot.org, CTLA4 is expressed in lymph node, lung, lymphocyte, among other tissues. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2018-05-30
Question
My lab would like to test anti-CTLA4 (CD152) Monoclonal antibody M00020 on human lymphocyte for research purposes, then I may be interested in using anti-CTLA4 (CD152) Monoclonal antibody M00020 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Jones
Verified customer
Asked: 2017-12-20
Answer
The products we sell, including anti-CTLA4 (CD152) Monoclonal antibody M00020, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-20
Question
Is a blocking peptide available for product anti-CTLA4 (CD152) Monoclonal antibody (M00020)?
T. Jackson
Verified customer
Asked: 2015-09-08
Answer
We do provide the blocking peptide for product anti-CTLA4 (CD152) Monoclonal antibody (M00020). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-09-08
Question
We were content with the WB result of your anti-CTLA4 (CD152) Monoclonal antibody. However we have seen positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is CTLA4 supposed to be expressed?
B. Edwards
Verified customer
Asked: 2013-09-19
Answer
From what I have seen in literature, lung does express CTLA4. Generally CTLA4 expresses in cell membrane. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2013-09-19
