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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Mouse CTLA4 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse CTLA4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ctla4 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CTLA4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0717)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A37-F162
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0717 is reactive to Ctla4 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0717 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0717-CAP||Anti-Mouse Ctla4 Pre-coated 96-well strip microplate||1|
|EK0717-ST||Mouse Ctla4 Standard||2 vials, 10 ng/tube|
|EK0717-DA||Mouse Ctla4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CTLA4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CTLA-4, Dilution ratio of 1:1, concentration in serum is around 200pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CTLA4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CTLA-4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ctla4 (Source: Uniprot.Org, NCBI)
Cytotoxic T-lymphocyte protein 4
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 Ctla4|Cd152, Ctla, Ctla-4, Ly-5, Ly-56|cytotoxic T-lymphocyte-associated protein 4|cytotoxic T-lymphocyte protein 4|CD152 antigen|cytotoxic T-lymphocyte-associated antigen 4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ctla4, check out the Ctla4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ctla4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0717
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