Click image to see more details
Product Info Summary
| SKU: | PA1698-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYP11A1 Antibody
SKU/Catalog Number
PA1698-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP11A1 Antibody catalog # PA1698-1. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP11A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1698-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP11A1, different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1698-1 is reactive to CYP11A1 in Mouse, Rat
Applications
PA1698-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
60.102kDa
Background of CYP11A1
CYP11A1 (Cytochrome p450, family 11, subfamily A, polypeptide 1), also called CYTOCHROME P450SCC, CYTOCHROME P450C11A1 or CYP11A, is a mitochondrial enzyme associated with the conversion of cholesterol to pregnenolone. CYP11A1 is a member of the cytochrome P450 superfamily of enzymes. The CYP11A1 gene is mapped on 15q24.1. Expression of the CYP11A1 gene may play a role in skin physiology and pathology and that cutaneous proopiomelanocortin activity may be autoregulated by a feedback mechanism involving glucocorticoids synthesized locally by Slominski et al. Using in vitro studies, CYP11A1 catalyzed the side-chain cleavage of 7-dehydrocholesterol to form 7-dehydropregnenolone. In addition, CYP11A1 catalyzed the metabolism of biologically inert vitamin D3, which is formed from 7-dehydrocholesterol, to form 2 hydroxylated products, 20-hydroxyvitamin D3 and 20, 22-dihydroxyvitamin D3. Mutations in the CYP11A1 gene cause congenital adrenal insufficiency with partial or complete 46, XY sex reversal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-CYP11A1 antibody, PA1698-1, Western blotting
All lanes: Anti CYP11A1 (PA1698-1) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 60KD
Observed bind size: 60KD
Protein Target Info & Infographic
Gene/Protein Information For CYP11A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP11A1
Full Name
Cholesterol side-chain cleavage enzyme, mitochondrial
Weight
60.102kDa
Superfamily
cytochrome P450 family
Alternative Names
Cholesterol desmolase; cholesterol monooxygenase (side-chain-cleaving); cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; Cytochrome P450 11A1; Cytochrome P450(scc); cytochrome P450, family 11, subfamily A, polypeptide 1; cytochrome P450C11A1; EC 1.14.15; EC 1.14.15.6; steroid 20-22-lyase; subfamily XIA (cholesterol side chain cleavage) CYP11A1 CYP11A, CYPXIA1, P450SCC cytochrome P450 family 11 subfamily A member 1 cholesterol side-chain cleavage enzyme, mitochondrial|cholesterol 20-22 desmolase|cholesterol monooxygenase (side-chain cleaving)|cytochrome P450 11A1|cytochrome P450 family 11 subfamily A polypeptide 1|cytochrome P450(scc)|cytochrome P450, subfamily XIA (cholesterol side chain cleavage)|steroid 20-22-lyase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP11A1, check out the CYP11A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP11A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP11A1 Antibody (PA1698-1)
Hello CJ!
PA1698-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Abnormal Apoptosis of Trophoblastic Cells Is Related to the Up-Regulation of CYP11A Gene in Placenta of Preeclampsia Patients
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CYP11A1 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right adrenal gland using anti-CYP11A1 antibody PA1698-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-16
Question
Will anti-CYP11A1 antibody PA1698-1 work for WB with right adrenal gland?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of right adrenal gland, CYP11A1 is highly expressed in right adrenal gland. So, it is likely that anti-CYP11A1 antibody PA1698-1 will work for WB with right adrenal gland.
Boster Scientific Support
Answered: 2020-01-03
Question
I am interested in to test anti-CYP11A1 antibody PA1698-1 on mouse right adrenal gland for research purposes, then I may be interested in using anti-CYP11A1 antibody PA1698-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The products we sell, including anti-CYP11A1 antibody PA1698-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-28
Question
Is a blocking peptide available for product anti-CYP11A1 antibody (PA1698-1)?
T. Kulkarni
Verified customer
Asked: 2019-08-28
Answer
We do provide the blocking peptide for product anti-CYP11A1 antibody (PA1698-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-28
Question
I have a question about product PA1698-1, anti-CYP11A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1698-1 anti-CYP11A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-12
Question
We are currently using anti-CYP11A1 antibody PA1698-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-CYP11A1 antibody (PA1698-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
I was wanting to use your anti-CYP11A1 antibody for WB for mouse right adrenal gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right adrenal gland identification?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
It shows on the product datasheet, PA1698-1 anti-CYP11A1 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse right adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-05
Question
I have attached the WB image, lot number and protocol we used for right adrenal gland using anti-CYP11A1 antibody PA1698-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-30
Question
Is this PA1698-1 anti-CYP11A1 antibody reactive to the isotypes of CYP11A1?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
The immunogen of PA1698-1 anti-CYP11A1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP11A1(53-66aa DNGWLNLYHFWRES), different from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-23
Question
Does anti-CYP11A1 antibody PA1698-1 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
Our lab technicians have not tested anti-CYP11A1 antibody PA1698-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CYP11A1 antibody PA1698-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-06
Question
We need using your anti-CYP11A1 antibody for r-mmu-196108; pregnenolone biosynthesis studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
I appreciate your inquiry. This PA1698-1 anti-CYP11A1 antibody is validated on rat testis tissue, tissue lysate, mouse brain, brain tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-11
Question
Would PA1698-1 anti-CYP11A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
It shows on the product datasheet, PA1698-1 anti-CYP11A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-05
Question
Is there a BSA free version of anti-CYP11A1 antibody PA1698-1 available?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CYP11A1 antibody PA1698-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-09
Question
My boss were well pleased with the WB result of your anti-CYP11A1 antibody. However we have seen positive staining in brain mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is CYP11A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
From literature, brain does express CYP11A1. Generally CYP11A1 expresses in mitochondrion inner membrane. Regarding which tissues have CYP11A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Choriocarcinoma, Pubmed ID: 1849407
Placenta, Pubmed ID: 3038854
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-18
Question
I see that the anti-CYP11A1 antibody PA1698-1 works with WB, what is the protocol used to produce the result images on the product page?
D. Krishna
Verified customer
Asked: 2016-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-16
Question
We have been able to see staining in mouse placenta testis. Are there any suggestions? Is anti-CYP11A1 antibody supposed to stain placenta testis positively?
J. Parker
Verified customer
Asked: 2015-03-12
Answer
From literature placenta testis does express CYP11A1. From Uniprot.org, CYP11A1 is expressed in right adrenal gland, placenta, placenta testis, brain, choriocarcinoma, among other tissues. Regarding which tissues have CYP11A1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Choriocarcinoma, Pubmed ID: 1849407
Placenta, Pubmed ID: 3038854
Placenta, and Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-03-12
