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Product Info Summary
| SKU: | A05393 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CysLTR1/Cyslt1 Antibody
SKU/Catalog Number
A05393
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CysLTR1/Cyslt1 Antibody catalog # A05393. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CysLTR1/Cyslt1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05393)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CYSLTR1. AA range:131-180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05393 is reactive to CYSLTR1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05393 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of HeLa cells using CysLTR1 Polyclonal Antibody
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Western blot analysis of lysates from COLO205, HT-29, and HeLa cells, using CYSLTR1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using CYSLTR1 antibody.
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Immunofluorescence analysis of COS7 cells, using CYSLTR1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CysLTR1/Cyslt1 Antibody (A05393)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CysLTR1/Cyslt1 Antibody
Question
Is there a BSA free version of anti-CysLTR1/Cyslt1 antibody A05393 available?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CysLTR1/Cyslt1 antibody A05393 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-26
Question
My question regarding product A05393, anti-CysLTR1/Cyslt1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05393 anti-CysLTR1/Cyslt1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this A05393 anti-CysLTR1/Cyslt1 antibody reactive to the isotypes of CYSLTR1?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The immunogen of A05393 anti-CysLTR1/Cyslt1 antibody is Synthesized peptide derived from the Internal region of human CysLTR1. at AA rangle: 100-180. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-CysLTR1/Cyslt1 antibody A05393 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The anti-CysLTR1/Cyslt1 antibody (A05393) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-12
Question
I am interested in to test anti-CysLTR1/Cyslt1 antibody A05393 on human tonsil for research purposes, then I may be interested in using anti-CysLTR1/Cyslt1 antibody A05393 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
The products we sell, including anti-CysLTR1/Cyslt1 antibody A05393, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-10
Question
I was wanting to use your anti-CysLTR1/Cyslt1 antibody for WB for human tonsil on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tonsil identification?
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
As indicated on the product datasheet, A05393 anti-CysLTR1/Cyslt1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tonsil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tonsil using anti-CysLTR1/Cyslt1 antibody A05393. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-08
