Anti-5 Lipoxygenase/ALOX5 Antibody
Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human.
Anti-5 Lipoxygenase/ALOX5 Antibody Info At A Glance
|Product Name||Anti-5 Lipoxygenase/ALOX5 Antibody
See all 5-Lipoxygenase primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for 5 LO/ALOX5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. 5 LO/ALOX5 information: Molecular Weight: 77983 MW; Subcellular Localization: Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.|
|Cite This Product||Anti-5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1644)|
|Similar Products From
|Anti-5 Lipoxygenase/ALOX5 Antibody may replace the following items: sc 135685|sc 136195|sc 20785|sc 8885|sc 8886.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5 (650-667aa AERNKKKQLPYYYLSPDR), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For ALOX5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||240|
|Species Of This Entry||Human|
|Protein Name||Arachidonate 5-lipoxygenase|
|Alternative Names||5-Lipoxygenase|5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 18.104.22.168; leukotriene A4 synthase; LOG5; LOX5A; MGC163204|
|Post Tranlational Modifications||Phosphoprotein|
|Subcellular Localization||Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.|
|Gene Location||10q11.21, on Chromasome 10, gene sequence: NC_000010.11 (45374166..45446121)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ALOX5 (Source: Uniprot.org, NCBI)
|Protein Function||Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.|
|Interacting Proteins||CEP63; COTL1; FGR; GATC; HCK; HHIPL2; MAD1L1; MEOX1; NUTM1; PRKACA; REL; SYCE1; YES1|
|Research Areas||Cancer; Cholesterol Metabolism; Lipid and Metabolism; Signal Transduction|
|Related Diseases||Asthma; Inflammation; Malignant Neoplasms; Atherosclerosis; Myeloid Leukemia; Leukemia; Neoplasms; Cardiovascular Diseases; Myeloid Leukemia, Chronic; Dysequilibrium Syndrome; Infarction; Rhinorrhea; Myocardial Infarction; Alzheimer's Disease; Coronary Artery Disease; Carcinogenesis; Cerebrovascular Accident; Urticaria; Hyperplasia; Malignant Tumor Of Colon|
|Related Pathways||Pathogenesis; Secretion; Cell Proliferation; Rna Interference; Hypersensitivity; Cell Cycle; Cell Death; Lipoxygenase Pathway; Angiogenesis; Transport; Gene Silencing; Cellular Senescence; Monocyte Chemotaxis; Pigmentation; Muscle Contraction; Cell Division; Reverse Transcription; Macroautophagy; Chemotaxis; Mrna Transcription|
|Background||ALOX5(ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO(5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences(NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.|
|Scientific References||PMID: 3422434 by Dixon R.A.F., et al. Cloning of the cDNA for human 5-lipoxygenase.|
PMID: 2829172 by Matsumoto T., et al. Molecular cloning and amino acid sequence of human 5-lipoxygenase.
Our Boster Quality Guarantee for Anti-5 Lipoxygenase/ALOX5 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti ALOX5 (PA1644) at 0.5ug/ml
Lane 1: SW620 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
IHC(P): Human Lung Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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