Anti-5 Lipoxygenase/ALOX5 Antibody

5-Lipoxygenase antibody

Boster Bio Anti-5 Lipoxygenase/ALOX5 Antibody catalog # RP1031. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: RP1031
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-5 Lipoxygenase/ALOX5 Antibody

View all 5-Lipoxygenase Antibodies

SKU/Catalog Number

RP1031

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-5 Lipoxygenase/ALOX5 Antibody catalog # RP1031. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-5 Lipoxygenase/ALOX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1031)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1031 is reactive to ALOX5 in Human, Mouse, Rat

Applications

RP1031 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

77.983kDa

Background of 5-Lipoxygenase

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALOX5 (Source: Uniprot.org, NCBI)

Gene Name

ALOX5

Full Name

Arachidonate 5-lipoxygenase

Weight

77.983kDa

Superfamily

lipoxygenase family

Alternative Names

5Lipoxygenase; 5-Lipoxygenase; 5-LO; 5-LOX; 5LPG; ALOX5; arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; EC 1.13.11; EC 1.13.11.34; leukotriene A4 synthase; LOG5; LOX5A; MGC163204 ALOX5 5-LO, 5-LOX, 5LPG, LOG5 arachidonate 5-lipoxygenase polyunsaturated fatty acid 5-lipoxygenase|LOX-5|arachidonic acid 5-lipoxygenase|leukotriene A4 synthase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALOX5, check out the ALOX5 Infographic

ALOX5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALOX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-5 Lipoxygenase/ALOX5 Antibody

Question

We are currently using anti-5 Lipoxygenase/ALOX5 antibody RP1031 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

The anti-5 Lipoxygenase/ALOX5 antibody (RP1031) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-06

Size

Conjugation

Total: $370

SKU:RP1031

In stock, 1 left.

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RP1031
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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