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- Table of Contents
CTH antibody (PA1556) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of JK cells using anti-CTH antibody (PA1556).
Overlay histogram showing JK cells stained with PA1556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTH Antibody (PA1556, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing JK cells stained with PA1556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTH Antibody (PA1556, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Cystathionase/CTH Antibody Picoband®
CTH antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
CTH antibody was positively detected by WB in:
human K562 whole cell, human HepG2 whole cell, human THP-1 whole cell, human HCCT tissue, rat liver tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTH antibody was positively detected by ICC/IF in:
A431 cell
CTH antibody was positively detected by FC in:
JK cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Monkey
Predicted
Rabbit
Predicted
Product Information
Product Name
Anti-Cystathionase/CTH Antibody Picoband®
View all CTH Antibodies
View all CTH Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cystathionase, different from the related mouse and rat sequences by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
44.5 kDa
Background of CTH
Cystathionine gamma-lyase (or cystathionase) is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. The International Radiation Hybrid Mapping Consortium mapped the CTH gene to chromosome 1. The CTH gene had earlier been assigned to chromosome 16 by study of somatic cell hybrids. It is demonstrated that hydrogen sulfide (H2S) is physiologically generated by CTH.
Cite This Product
Anti-Cystathionase/CTH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1556)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Rat
88%
Moderate Homology
Sheep
88%
Moderate Homology
Mouse
81%
Moderate Homology
Cow
81%
Moderate Homology
Dog
81%
Moderate Homology
Zebrafish
69%
Low homology
Drosophila
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CTH antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CTH antibody
Flow Cytometry (FC) protocol for CTH antibody
Publications
Application:
Species:
Role of the 3-mercaptopyruvate sulfurtransferase in colon/colorectal cancers
Published: 2024-04-15
Journal: EUROPEAN JOURNAL OF CELL BIOLOGY
Application: WB
Impact Factor: 4.50
Species: Human
PMID: 38631098
Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma
Published: 2021-02-25
Journal: Frontiers in Oncology
Application: IHC
Impact Factor: 6.24
Species: Human
PMID: 33718182
MicroRNA-210 promotes angiogenesis in acute myocardial infarction
Published: 2018-02-20
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.92
Species: Rat
PMID: 29484401
CXCL5 as an autocrine or paracrine cytokine is associated with proliferation and migration of hepatoblastoma HepG2 cells
Published: 2017-10-20
Journal: Oncology Letters
Application: WB
Impact Factor: 1.39
Species: Human
PMID: 29344240
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Product Promise
We promise Anti-Cystathionase/CTH Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1556
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026