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Product Info Summary
| SKU: | EK1120 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human CCL15/Mip 1 Delta ELISA Kit PicoKine®
View all CCL15/MIP-1 delta ELISA kits
SKU/Catalog Number
EK1120
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CCL15/Mip 1 Delta ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CCL15/Mip 1 Delta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1120)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Q22-I113
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1120 is reactive to CCL15 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1120 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL15/MIP-1 delta
Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1120-CAP | Anti-Human CCL15 Pre-coated 96-well strip microplate | 1 |
| EK1120-ST | Human CCL15 Standard | 2 vials, 10 ng/tube |
| EK1120-DA | Human CCL15 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.042 | 0.092 | 0.121 | 0.194 | 0.319 | 0.570 | 1.023 | 2.010 |
Data Example Images
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Human CCL15 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CCL15/Mip 1 Delta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 185 | 439 | 31 | 188 | 417 |
| Standard deviation | 2.31 | 9.8 | 31.6 | 2.75 | 13.34 | 37.11 |
| CV (%) | 7% | 5.3% | 7.2% | 8.9% | 7.1% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL15/MIP-1 delta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 29 | 29 | 33 | 31 | 2 | 6.4% |
| Sample 2 | 185 | 185 | 181 | 184 | 183 | 1.63 | 0.8% |
| Sample 3 | 439 | 430 | 478 | 439 | 446 | 18.55 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For CCL15 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q16663
Gene ID
6359
Gene Name
CCL15
Full Name
C-C motif chemokine 15
Weight
12.248kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15 CCL15 HCC-2, HMRP-2B, LKN-1, LKN1, MIP-1 delta, MIP-1D, MIP-5, MRP-2B, NCC-3, NCC3, SCYA15, SCYL3, SY15 C-C motif chemokine ligand 15 C-C motif chemokine 15|chemokine (C-C motif) ligand 15|chemokine CC-2|leukotactin 1|macrophage inflammatory protein 5|new CC chemokine 3|small inducible cytokine subfamily A (Cys-Cys), member 15|small-inducible cytokine A15
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL15, check out the CCL15 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL15: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human CCL15/Mip 1 Delta ELISA Kit PicoKine® (EK1120)
No publications found for EK1120
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Human CCL15/Mip 1 Delta ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated CCL15 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL15 antigen?
R. Baker
Verified customer
Asked: 2020-06-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-11
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Jha
Verified customer
Asked: 2020-04-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-04-02
Question
Q: can you suggest the dilution ratio of serum samples for detection of CCL15 in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL15 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-07-04
Question
Q: how can I thaw whole blood sample for CCL15 ELISA after freezing?
W. Patel
Verified customer
Asked: 2018-11-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL15 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: can I use citrate plasma as samples in Human CCL15 Picokine® ELISA Kit (Catalog # EK1120)?
C. Scott
Verified customer
Asked: 2018-05-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CCL15 causing degradation of its protein structure. CCL15 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CCL15 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-24
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
R. Patel
Verified customer
Asked: 2015-09-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-09-22
