Human CCL15/Mip 1 Delta PicoKine™ ELISA Kit
SKU | EK1120 |
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Size | 96wells/kit, with removable strips. |
Reactivity | Human |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
Sensitivity | <10pg/ml |
Assay Range | 15.6pg/ml-1000pg/ml |
Overview
Product Name | Human CCL15/Mip 1 Delta PicoKine™ ELISA Kit |
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SKU/Catalog Number | EK1120 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL15. 96wells/kit, with removable strips. |
Cite This Product | Human CCL15/Mip 1 Delta PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1120) |
Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)..
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <10pg/ml |
Assay Range | 15.6pg/ml-1000pg/ml |
Immunogen | Expression system for standard: E.coli; Immunogen sequence: Q22-I113 |
Reactivity | Human |
Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Antibody Clonalities | Capture Antibody | Detection Antibody: monoclonal antibody from mouse|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK1120-CAP | 96-well plate precoated with anti-Human CCL15 antibody | 1 |
EK1120-ST | lyophilized recombinant Human CCL15 standard | 10ng/tube |
EK1120-DA | biotinylated anti-Human CCL15 antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human CCL15/Mip 1 Delta PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.042 | 0.092 | 0.121 | 0.194 | 0.319 | 0.570 | 1.023 | 2.010 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 185 | 439 | 31 | 188 | 417 |
Standard deviation | 2.31 | 9.8 | 31.6 | 2.75 | 13.34 | 37.11 |
CV(%) | 7% | 5.3% | 7.2% | 8.9% | 7.1% | 8.9% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 33 | 29 | 29 | 33 | 31 | 2 | 6.4% |
Sample 2 | 185 | 185 | 181 | 184 | 183 | 1.63 | 0.8% |
Sample 3 | 439 | 430 | 478 | 439 | 446 | 18.55 | 4.1% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | Q16663 |
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Gene Name | CCL15 |
Protein Name | C-C motif chemokine 15 |
Tissue Specificity | Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients. . |
Alternative Names | C-C motif chemokine 15;Chemokine CC-2;HCC-2;Leukotactin-1;LKN-1;MIP-1 delta;Macrophage inflammatory protein 5;MIP-5;Mrp-2b;NCC-3;Small-inducible cytokine A15;CCL15(22-92);CCL15(25-92);CCL15(29-92);CCL15;MIP5, NCC3, SCYA15; |
Subcellular Localization | Secreted. |
Molecular Weight | 12248 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Protein Function | Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. . |
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Research Areas | Angiogenesis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm. *You can search these to find other products in these research areas. |
Background | Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14. |
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Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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