Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody

Product Name

Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody

View all Cytochrome c Antibodies

SKU/Catalog Number

M03529-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody catalog # M03529-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GOC-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cytochrome C

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03529-2 is reactive to Cycs in Human, Mouse, Rat

Applications

M03529-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

11.605kDa

Background of Cytochrome c

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CYCS using anti-CYCS antibody (M03529-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HT-1080 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat skeletal muscle tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS antigen affinity purified monoclonal antibody (Catalog # M03529-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS at approximately 14 kDa. The expected band size for CYCS is at 12 kDa.

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Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For Cycs (Source: Uniprot.org, NCBI)

Alternative Names

CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4 Cycs|cytochrome c, somatic|cytochrome c, somatic

For more info on Cycs, check out the Cycs Infographic

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In this infographic, you will see the following information for Cycs: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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