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Product Info Summary
| SKU: | M03529-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody
SKU/Catalog Number
M03529-2
BM4571 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody catalog # M03529-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03529-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GOC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome C
Reactive Species
M03529-2 is reactive to CYCS in Human, Mouse, Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
11.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03529-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
Positive Control
WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human HT-1080 whole cell, rat kidney tissue, rat skeletal muscle tissue, mouse kidney tissue, mouse skeletal muscle tissue
IHC: human liver tissue
Validation Images & Assay Conditions
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Western blot analysis of CYCS using anti-CYCS antibody (M03529-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human HT-1080 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat skeletal muscle tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS antigen affinity purified monoclonal antibody (Catalog # M03529-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS at approximately 14 kDa. The expected band size for CYCS is at 12 kDa.
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Immunohistochemical analysis of paraffin-embedded human liver, using Cytochrome C Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using Cytochrome C Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody (M03529-2)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Cytochrome C CYCS Rabbit Monoclonal Antibody
Question
We purchased anti-Cytochrome C Rabbit Monoclonal antibody for IP on kidney in a previous project. I am using human, and I plan to use the antibody for IF next. I am interested in examining kidney as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
I viewed the website and datasheets of our anti-Cytochrome C Rabbit Monoclonal antibody and it seems that M03529-2 has been validated on human in both IP and IF. Thus M03529-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-Cytochrome C Rabbit Monoclonal antibody M03529-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-Cytochrome C Rabbit Monoclonal antibody (M03529-2) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
We have been able to see staining in human erythroleukemia. Any tips? Is anti-Cytochrome C Rabbit Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
Based on literature erythroleukemia does express CYCS. Based on Uniprot.org, CYCS is expressed in heart, cerebellum, amygdala, bone marrow, brain, kidney, lung, skeletal muscle, skin,
rc testis urinary bladder, erythroleukemia, liver, among other tissues. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-08-30
Question
Is this M03529-2 anti-Cytochrome C Rabbit Monoclonal antibody reactive to the isotypes of CYCS?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
The immunogen of M03529-2 anti-Cytochrome C Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytochrome C . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-20
Question
My team were well pleased with the WB result of your anti-Cytochrome C Rabbit Monoclonal antibody. However we have observed positive staining in brain mitochondrion intermembrane space. using this antibody. Is that expected? Could you tell me where is CYCS supposed to be expressed?
L. Miller
Verified customer
Asked: 2015-01-29
Answer
From what I have seen in literature, brain does express CYCS. Generally CYCS expresses in mitochondrion intermembrane space. Regarding which tissues have CYCS expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Bone marrow, Brain, Kidney, Lung, Skeletal muscle, Skin, Testis, and Urinary bladder, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Erythroleukemia, Pubmed ID: 23186163
Heart, Pubmed ID: 13933734, 14063298, 9515723
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-01-29
