Anti-Smac/Diablo Antibody Picoband™

Boster Bio Anti-Smac/Diablo Antibody Picoband™ catalog # PB9860. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9860
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-Smac/Diablo Antibody Picoband™

See all SMAC/Diablo products

SKU/Catalog Number

PB9860

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Smac/Diablo Antibody Picoband™ catalog # PB9860. Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smac/Diablo Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9860)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

PB9860 is reactive to DIABLO in Human, Mouse, Rat

Applications

PB9860 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
ELISA , 0.1-0.5μg/ml, Human, -

Validation Images & Assay Conditions

Gene/Protein Information For DIABLO (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NR28

Gene ID

56616

Gene Name

DIABLO

Full Name

Diablo homolog, mitochondrial

Weight

27.131kDa

Alternative Names

0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial DIABLO DFNA64, SMAC diablo IAP-binding mitochondrial protein diablo homolog, mitochondrial|diablo-like protein|direct IAP-binding protein with low pI|second mitochondria-derived activator of caspase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DIABLO, check out the DIABLO Infographic

DIABLO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DIABLO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9860 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

You F, Li Q, Jin G, Zheng Y, Chen J, Yang H. BMC Neurosci. 2017 Jan 12;18(1):12. doi: 10.1186/s12868-016-0329-9. Genistein protects against Aβ25–35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers

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3 Customer Q&As for Anti-Smac/Diablo Antibody Picoband™

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-Smac/Diablo antibody PB9860. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

Will anti-Smac/Diablo antibody PB9860 work for IHC with right testis?

Verified Customer

Verified customer

Asked: 2018-02-09

Answer

According to the expression profile of right testis, DIABLO is highly expressed in right testis. So, it is likely that anti-Smac/Diablo antibody PB9860 will work for IHC with right testis.

Boster Scientific Support

Answered: 2018-02-09

Question

We are currently using anti-Smac/Diablo antibody PB9860 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

G. Thomas

Verified customer

Asked: 2015-07-16

Answer

The anti-Smac/Diablo antibody (PB9860) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-16

Conjugation

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