Product Info Summary
SKU: | A01432-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 14 KRT14 Antibody
View all Cytokeratin 14 Antibodies
SKU/Catalog Number
A01432-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 14 KRT14 Antibody catalog # A01432-2. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 14 KRT14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01432-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Elk-1 around the phosphorylation site of S389.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01432-2 is reactive to KRT14 in Human, Mouse, Rat
Applications
A01432-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
51.561kDa
Background of Cytokeratin 14
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104.
Marais R, et al. (1993) Cell 73:381-393.
Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824.
Hill C S, et al. (1995) Cell. 80:199-211.
Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of KRT14 using Anti-Cytokeratin 14 KRT14 Antibody (A01432-2).
ICC staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Cytokeratin 14 KRT14 Antibody A01432-2
Western blot analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For KRT14 (Source: Uniprot.org, NCBI)
Gene Name
KRT14
Full Name
Keratin, type I cytoskeletal 14
Weight
51.561kDa
Superfamily
intermediate filament family
Alternative Names
CK14; CK-14; Cytokeratin 14; cytokeratin-14; EBS3; EBS4; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; keratin, type I cytoskeletal 14; keratin-14; KRT14; NFJ KRT14 CK14, EBS3, EBS4, K14, NFJ keratin 14 keratin, type I cytoskeletal 14|cytokeratin 14|keratin 14, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT14, check out the KRT14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 14 KRT14 Antibody (A01432-2)
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No publications found for A01432-2
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