Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine™

Human TPP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TPP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2042
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine™

See all Tripeptidyl-Peptidase I/TPP1 products

SKU/Catalog Number

EK2042

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TPP1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TPP1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2042)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L196-P563

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2042 is reactive to TPP1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK2042 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK2042-CAP Anti-Human TPP1 Pre-coated 96-well strip microplate 1
EK2042-ST Human TPP1 Standard 2 vials, 10 ng/tube
EK2042-DA Human TPP1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0370.1060.1810.3100.5580.9431.5522.170

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2721160394630311874281
Standard deviation10.8854.52205.1915.4570.03303.95
CV (%)4.0%4.7%5.2%5.1%5.9%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tripeptidyl-Peptidase I/TPP1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127228828531529015.635.3%
Sample 2116010881127980108867.766.2%
Sample 339464041393145464116251.816.1%
*number of samples for each test n=16.

Gene/Protein Information For TPP1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14773

Gene ID

1200

Gene Name

TPP1

Full Name

Tripeptidyl-peptidase 1

Weight

61.248kDa

Alternative Names

Cell growth-inhibiting gene 1 protein; ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease); CLN2; CLN2EC 3.4.14.9; growth-inhibiting protein 1; LINCL; LPIC; lysosomal pepstatin insensitive protease; Lysosomal pepstatin-insensitive protease; MGC21297; TPP1; TPP-1; TPP-I; Tripeptidyl aminopeptidase; tripeptidyl peptidase I; tripeptidyl-peptidase 1; TripeptidylPeptidase I; Tripeptidyl-Peptidase I TPP1 CLN2, GIG1, LPIC, SCAR7, TPP-1 tripeptidyl peptidase 1 tripeptidyl-peptidase 1|cell growth-inhibiting gene 1 protein|growth-inhibiting protein 1|lysosomal pepstatin insensitive protease|lysosomal pepstatin-insensitive carboxypeptidase|tripeptidyl aminopeptidase|tripeptidyl peptidase I

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TPP1, check out the TPP1 Infographic

TPP1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TPP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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10 Customer Q&As for Human TPP1/Tripeptidyl-peptidase 1 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for TPP1 ELISA kit? I performed your TPP1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TPP1 even though the O.D. values are not very high?

Z. Harris

Verified customer

Asked: 2020-10-19

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TPP1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of TPP1 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2020-10-19

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-10-03

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-10-03

Question

Q: which procedure should I follow in order to thaw whole blood sample for TPP1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TPP1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-02-21

Question

Q: is it okay to use citrate plasma as samples in Human TPP1 Picokine® ELISA Kit (Catalog # EK2042)?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TPP1 causing disruption of its protein structure. TPP1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TPP1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-01-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Chakrabarti

Verified customer

Asked: 2019-10-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-10-14

Question

Q: how to analyze ELISA data? I have performed TPP1 level in plasma.

Verified Customer

Verified customer

Asked: 2019-04-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-04-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

B. Varma

Verified customer

Asked: 2018-08-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-08-14

Question

Q: Can TPP1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-09-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-09-23

Question

Q: if the enzyme conjugated TPP1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TPP1 antigen?

Verified Customer

Verified customer

Asked: 2017-02-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-02-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-01-23

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-01-23

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