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Product Info Summary
| SKU: | M01432-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody
View all Cytokeratin 14 Antibodies
SKU/Catalog Number
M01432-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody catalog # M01432-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01432-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FEF-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 14
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01432-2 is reactive to KRT14 in Human, Mouse, Rat
Applications
M01432-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
51.561kDa
Background of Cytokeratin 14
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human stomach, using Cytokeratin 14 Antibody.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HACAT cells, using Cytokeratin 14 Antibody.
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Western blot analysis of Cytokeratin 14 expression in A431 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For KRT14 (Source: Uniprot.org, NCBI)
Gene Name
KRT14
Full Name
Keratin, type I cytoskeletal 14
Weight
51.561kDa
Superfamily
intermediate filament family
Alternative Names
CK14; CK-14; Cytokeratin 14; cytokeratin-14; EBS3; EBS4; K14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14; keratin, type I cytoskeletal 14; keratin-14; KRT14; NFJ KRT14 CK14, EBS3, EBS4, K14, NFJ keratin 14 keratin, type I cytoskeletal 14|cytokeratin 14|keratin 14, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT14, check out the KRT14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody (M01432-2)
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No publications found for M01432-2
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Cytokeratin 14 KRT14 Rabbit Monoclonal Antibody
Question
We have seen staining in human cervix carcinoma. Any tips? Is anti-Cytokeratin 14 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
From what I have seen in literature cervix carcinoma does express KRT14. From what I have seen in Uniprot.org, KRT14 is expressed in gingival epithelium, brain, pancreas skin, epidermis, lymphoblast, cervix carcinoma, liver, among other tissues. Regarding which tissues have KRT14 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Epidermis, Pubmed ID: 6186381
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2020-04-23
Question
I see that the anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-25
Question
Would anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 work for IF with brain?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to the expression profile of brain, KRT14 is highly expressed in brain. So, it is likely that anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 will work for IF with brain.
Boster Scientific Support
Answered: 2020-02-10
Question
Do you have a BSA free version of anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 available?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody reactive to the isotypes of KRT14?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The immunogen of M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytokeratin 14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-09
Question
I am interested in using your anti-Cytokeratin 14 Rabbit Monoclonal antibody for dowling-meara type (dm- studies. Has this antibody been tested with western blotting on hacat cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
We appreciate your inquiry. This M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody is tested on a431 cell lysate, hacat cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-05
Question
Will M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
You can see on the product datasheet, M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-26
Question
I have a question about product M01432-2, anti-Cytokeratin 14 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-24
Question
See below the WB image, lot number and protocol we used for brain using anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
you antibody to test anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 on mouse brain for research purposes, then I may be interested in using anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The products we sell, including anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-26
Question
I was wanting to use your anti-Cytokeratin 14 Rabbit Monoclonal antibody for IF for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
E. Wu
Verified customer
Asked: 2018-12-05
Answer
You can see on the product datasheet, M01432-2 anti-Cytokeratin 14 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-05
Question
We are currently using anti-Cytokeratin 14 Rabbit Monoclonal antibody M01432-2 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The anti-Cytokeratin 14 Rabbit Monoclonal antibody (M01432-2) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-23
Question
Is a blocking peptide available for product anti-Cytokeratin 14 Rabbit Monoclonal antibody (M01432-2)?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
We do provide the blocking peptide for product anti-Cytokeratin 14 Rabbit Monoclonal antibody (M01432-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-12-05
Question
We ordered your anti-Cytokeratin 14 Rabbit Monoclonal antibody for IHC on lymphoblast in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. I am looking for examining lymphoblast as well as epidermis in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
L. Brown
Verified customer
Asked: 2015-07-24
Answer
I viewed the website and datasheets of our anti-Cytokeratin 14 Rabbit Monoclonal antibody and I see that M01432-2 has been validated on mouse in both IHC and WB. Thus M01432-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-07-24
Question
My colleagues were content with the WB result of your anti-Cytokeratin 14 Rabbit Monoclonal antibody. However we have observed positive staining in lymphoblast cytoplasm. nucleus. note=expressed in both using this antibody. Is that expected? Could you tell me where is KRT14 supposed to be expressed?
E. Singh
Verified customer
Asked: 2014-12-18
Answer
From what I have seen in literature, lymphoblast does express KRT14. Generally KRT14 expresses in cytoplasm. nucleus. note=expressed in both. Regarding which tissues have KRT14 expression, here are a few articles citing expression in various tissues:
Brain, Pancreas, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Epidermis, Pubmed ID: 6186381
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2014-12-18
