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Product Info Summary
| SKU: | A07410 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cytokeratin 4 KRT4 Antibody
SKU/Catalog Number
A07410
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 4 KRT4 Antibody catalog # A07410. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 4 KRT4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07410)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 200-260
Reactive Species
A07410 is reactive to KRT4 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
56.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07410 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Western blot analysis of SKOV3 293T lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-Cytokeratin 4 KRT4 Antibody (A07410)
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Customer Q&As
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5 Customer Q&As for Anti-Cytokeratin 4 KRT4 Antibody
Question
Will A07410 anti-Cytokeratin 4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
It shows on the product datasheet, A07410 anti-Cytokeratin 4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
I would like to test anti-Cytokeratin 4 antibody A07410 on human tongue squamous epithelium for research purposes, then I may be interested in using anti-Cytokeratin 4 antibody A07410 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The products we sell, including anti-Cytokeratin 4 antibody A07410, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-18
Question
I have attached the WB image, lot number and protocol we used for tongue squamous epithelium using anti-Cytokeratin 4 antibody A07410. Please let me know if you require anything else.
D. Wu
Verified customer
Asked: 2019-04-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-02
Question
I have a question about product A07410, anti-Cytokeratin 4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07410 anti-Cytokeratin 4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-28
Question
Does anti-Cytokeratin 4 antibody A07410 work on bovine WB with brain ovary?
M. Mitchell
Verified customer
Asked: 2017-07-11
Answer
Our lab technicians have not tested anti-Cytokeratin 4 antibody A07410 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cytokeratin 4 antibody A07410 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-11
