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Product Info Summary
| SKU: | M00398-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226
KRT5" target="_blank" class="text-orange">View all KRT5 Antibodies
SKU/Catalog Number
M00398-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (Catalog # M00398-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00398-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM226
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of Human Cytokeratin 5
Cross-reactivity
This antibody reacts to Human Cytokeratin 5. This antibody may also react to bovine, mouse, or rat Cytokeratin, as predicted by immunogen homology.
Reactive Species
M00398-3 is reactive to KRT5 in Human
Calculated molecular weight
62.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00398-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100 dilution
WB: 1:1000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of A431 cell lysates using RM226 using Anti-CK-5 rabbit monoclonal antibody (Clone RM226) at a 1:1000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections.
Specific Publications For Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (M00398-3)
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4 Customer Q&As for Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226
Question
We want using your anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 for epidermolysis bullosa simplex studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your inquiry. This M00398-3 anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 is validated on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-20
Question
We have seen staining in human lymphoblast. What should we do? Is anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
According to literature lymphoblast does express KRT5. According to Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 M00398-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 (M00398-3) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
Question
We purchased anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone
D. Yang
Verified customer
Asked: 2016-08-23
Answer
I looked at the website and datasheets of our anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2016-08-23
