Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226

KRT5 antibody

Boster Bio Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (Catalog # M00398-3). Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00398-3
Size: 100ul
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226

View all KRT5 Antibodies

SKU/Catalog Number

M00398-3

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (Catalog # M00398-3). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00398-3)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM226

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the C-terminus of Human Cytokeratin 5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to Human Cytokeratin 5. This antibody may also react to bovine, mouse, or rat Cytokeratin, as predicted by immunogen homology.

Reactive Species

M00398-3 is reactive to KRT5 in Human

Applications

M00398-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

62.378kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:100 dilution
WB: 1:1000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For KRT5 (Source: Uniprot.org, NCBI)

Gene Name

KRT5

Full Name

Keratin, type II cytoskeletal 5

Weight

62.378kDa

Superfamily

intermediate filament family

Alternative Names

58 kDa cytokeratin; CK5; CK-5; cytokeratin-5; DDD; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes); keratin 5; keratin, type II cytoskeletal 5; Keratin-5; KRT5A; Type-II keratin Kb5 KRT5 CK5, DDD, DDD1, EBS2, K5A, KRT5 keratin 5 keratin, type II cytoskeletal 5|58 kda cytokeratin|CK-5|cytokeratin-5|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin 5, type II|type-II keratin Kb5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KRT5, check out the KRT5 Infographic

KRT5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KRT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Cytokeratin 5 Rabbit Monoclonal Antibody, Clone#RM226

Question

We want using your anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 for epidermolysis bullosa simplex studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

We appreciate your inquiry. This M00398-3 anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 is validated on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-20

Question

We have seen staining in human lymphoblast. What should we do? Is anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 supposed to stain lymphoblast positively?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

According to literature lymphoblast does express KRT5. According to Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843

Boster Scientific Support

Answered: 2019-07-29

Question

We are currently using anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 M00398-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

The anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone#RM226 (M00398-3) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-24

Question

We purchased anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone

D. Yang

Verified customer

Asked: 2016-08-23

Answer

I looked at the website and datasheets of our anti-Cytokeratin 5 Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2016-08-23

$431
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Lead time for this item is typically 1-2 weeks

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M00398-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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