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Product Info Summary
| SKU: | A00398 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 5/KRT5 Antibody Picoband®
SKU/Catalog Number
A00398
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytokeratin 5/KRT5 Antibody Picoband® catalog # A00398. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 5/KRT5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00398)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cytokeratin 5, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00398 is reactive to KRT5 in Human
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.4 kDa
Background of KRT5
Cytokeratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00398 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human A431 whole cell, human Hacat whole cell, human MCF-7 whole cell
IHC: human tonsil tissue, human oesophagus squama cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 5 antigen affinity purified polyclonal antibody (Catalog # A00398) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.
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IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398).
Cytokeratin 5 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytokeratin 5 Antibody (A00398) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398).
Cytokeratin 5 was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytokeratin 5 Antibody (A00398) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Cytokeratin 5/KRT5 Antibody Picoband® (A00398)
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4 Customer Q&As for Anti-Cytokeratin 5/KRT5 Antibody Picoband®
Question
We are currently using anti-Cytokeratin 5/KRT5 antibody A00398 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
The anti-Cytokeratin 5/KRT5 antibody (A00398) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01
Question
We purchased anti-Cytokeratin 5/KRT5 antibody for IHC on ectocervix in a previous experiment. I am using mouse, and We intend to use the antibody for WB next. We need examining ectocervix as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
I have checked the website and datasheets of our anti-Cytokeratin 5/KRT5 antibody and I see that A00398 has been validated on mouse in both IHC and WB. Thus A00398 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-26
Question
I am interested in using your anti-Cytokeratin 5/KRT5 antibody for autosomal recessive 1 studies. Has this antibody been tested with western blotting on human a431 whole cell lysates? We would like to see some validation images before ordering.
C. Li
Verified customer
Asked: 2015-07-02
Answer
I appreciate your inquiry. This A00398 anti-Cytokeratin 5/KRT5 antibody is validated on human a431 whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-07-02
Question
We have observed staining in mouse ectocervix. Any tips? Is anti-Cytokeratin 5/KRT5 antibody supposed to stain ectocervix positively?
K. Miller
Verified customer
Asked: 2013-07-08
Answer
According to literature ectocervix does express KRT5. According to Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2013-07-08
