Anti-DAP Kinase 2/DAPK2 Antibody
Rabbit IgG polyclonal antibody for Death-associated protein kinase 2(DAPK2) detection. Tested with WB in Human;Mouse;Rat.
Anti-DAP Kinase 2/DAPK2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-DAP Kinase 2/DAPK2 Antibody
See all DAP Kinase 2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for DAPK2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. DAPK2 information: Molecular Weight: 42898 MW; Subcellular Localization: Cytoplasm. Cytoplasmic vesicle, autophagosome lumen; Tissue Specificity: Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages.|
|Cite This Product||Anti-DAP Kinase 2/DAPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1558)|
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|Anti-DAP Kinase 2/DAPK2 Antibody may replace the following items: sc 130734|sc 100371|sc 10081|sc 10084.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human DAP Kinase 2(289-308aa DNQQAMVRRESVVNLENFRK), different from the related mouse and rat sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For DAPK2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||23604|
|Species Of This Entry||Human|
|Protein Name||Death-associated protein kinase 2|
|Superfamily||protein kinase superfamily|
|Alternative Names||DAP Kinase 2|DAP kinase 2; DAP-kinase-related protein 1 beta isoform; DAP-kinase-related protein 1; death-associated protein kinase 2; DRP1; DRP-1MGC119312; EC 2.7.11; EC 184.108.40.206|
|Post Tranlational Modifications||Phosphorylation; Methylation|
|Subcellular Localization||Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.|
|Gene Location||15q22.31, on Chromasome 15, gene sequence: NC_000015.10 (63907036..64046485, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For DAPK2 (Source: Uniprot.org, NCBI)
|Protein Function||Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).|
|Tissue Specificity||Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.|
|Interacting Proteins||ATF4; FAM9B|
|Research Areas||Apoptosis; Autophagy; Cellular Markers|
|Related Diseases||Neoplasms; Malignant Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Leukemia, Myelocytic, Acute; Myeloid Leukemia; Lymphoma; Hodgkin Disease; Leukemia; Stomach Neoplasms; Adenoma; Myeloid Leukemia, Chronic; Carcinoma; Parkinson Disease; Osteosarcoma; Multiple Myeloma; Severe Combined Immunodeficiency; Pituitary Diseases|
|Related Pathways||Cell Death; Neutrophil Differentiation; Regulation Of Macroautophagy; Macroautophagy; Cell Proliferation; Cellular Homeostasis; Biological Regulation; Pathogenesis; Catabolic Process|
|Background||Death-associated protein kinase 2 is an enzyme that in humans is encoded by the DAPK2 gene. This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1(DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. The DAPK2 mRNA may undergo alternative splicing to produce a DAPK3-like encoding transcript.|
|Scientific References||PMID: 10376525 by Kawai T., et al. Death-associated protein kinase 2 is a new calcium/calmodulin- dependent protein kinase that signals apoptosis through its catalytic activity.|
PMID: 10629061 by Inbal B., et al. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis.
Our Boster Quality Guarantee for Anti-DAP Kinase 2/DAPK2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti DAP Kinase 2 (PA1558) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 43KD
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