Product Info Summary
SKU: | M01145 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-DBT Mouse Monoclonal Antibody [Clone ID: OTI1G2]
SKU/Catalog Number
M01145
Size
100 µl
Description
Boster Bio DBT mouse monoclonal antibody, clone OTI1G2 (formerly 1G2). Catalog# M01145. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-DBT Mouse Monoclonal Antibody [Clone ID: OTI1G2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01145)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1G2
Isotype
IgG1
Immunogen
Full length human recombinant protein of human DBT (NP_001909) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01145 is reactive to DBT in Human, Mouse, Rat
Applications
M01145 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
53.487kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.
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HEK293T cells transfected with either DBT (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DBT antibody (M01145)
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Anti-DBT mouse monoclonal antibody (M01145) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.
Protein Target Info & Infographic
Gene/Protein Information For DBT (Source: Uniprot.org, NCBI)
Gene Name
DBT
Full Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Weight
53.487kDa
Superfamily
2-oxoacid dehydrogenase family
Alternative Names
BCATE2BCKAD E2 subunit; BCKADE2; BCKAD-E2; branched chain acyltransferase, E2 component; Branched-chain alpha-keto acid dehydrogenase complex component E2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex; dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease); dihydrolipoamide branched chain transacylase E2; Dihydrolipoamide branched chain transacylase; dihydrolipoyl transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase; E2 component of branche DBT BCATE2, BCKAD-E2, BCKADE2, BCKDH-E2, BCOADC-E2, E2, E2B dihydrolipoamide branched chain transacylase E2 lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial|52 kDa mitochondrial autoantigen of primary biliary cirrhosis|BCKAD E2 subunit|E2 component of branched chain alpha-keto acid dehydrogenase complex|branched chain 2-oxo-acid dehydrogenase complex component E2|branched chain acyltransferase, E2 component|branched-chain alpha-keto acid dehydrogenase complex component E2|dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex|dihydrolipoyl transacylase|dihydrolipoyllysine-residue (2-methylpropanoyl)transferase|lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex|mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DBT, check out the DBT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DBT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DBT Mouse Monoclonal Antibody [Clone ID: OTI1G2] (M01145)
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