Anti-Nestin Antibody Picoband™

Nestin antibody

Boster Bio Anti-Nestin Antibody Picoband™ catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9874
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Nestin Antibody Picoband™

View all Nestin Antibodies

SKU/Catalog Number

PB9874

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nestin Antibody Picoband™ catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nestin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9874)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9874 is reactive to NES in Human, Mouse, Rat

Applications

PB9874 is guaranteed for IHC, WB Boster Guarantee

Background of Nestin

Nestin is a type VI intermediate filament  (IF) protein that in humans is encoded by the NES gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α-internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For NES (Source: Uniprot.org, NCBI)

Uniprot ID

P48681

Gene ID

10763

Gene Name

NES

Full Name

Nestin

Weight

177.439kDa

Superfamily

intermediate filament family

Alternative Names

FLJ21841; NES; Nestin NES Nbla00170 nestin nestin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NES, check out the NES Infographic

NES infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PB9874 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Identification and differentiation therapy strategy of pterygium in vitro

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation

Tumorigenesis of nuclear transfer-derived embryonic stem cells is reduced through differentiation and enrichment following transplantation in the infarcted rat heart

Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons

Effects of 17β-estradiol pre-treated adult neural stem cells on neuronal differentiation and neurological recovery in rats with cerebral ischemia

Impaired Spatial Learning and Memory after Sevoflurane–Nitrous Oxide Anesthesia in Aged Rats Is Associated with Down-Regulated cAMP/CREB Signaling

Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

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4 Customer Q&As for Anti-Nestin Antibody Picoband™

Question

We are currently using anti-Nestin antibody PB9874 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

The anti-Nestin antibody (PB9874) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-01

Question

My lab would like using your anti-Nestin antibody for stem cell proliferation studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

Thank you for your inquiry. This PB9874 anti-Nestin antibody is validated on hela whole cell lysates, hepg2 whole cell lysates, rat kidney tissue, mouse kidney tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

We have seen staining in mouse cervix carcinoma. Are there any suggestions? Is anti-Nestin antibody supposed to stain cervix carcinoma positively?

C. Krishna

Verified customer

Asked: 2017-12-05

Answer

Based on literature cervix carcinoma does express NES. Based on Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414

Boster Scientific Support

Answered: 2017-12-05

Question

We have tried in the past anti-Nestin antibody for WB on metanephric glomerulus a few years ago. I am using mouse, and We intend to use the antibody for IHC next. My question regards examining metanephric glomerulus as well as neuroblastoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

K. Banerjee

Verified customer

Asked: 2017-08-15

Answer

I have checked the website and datasheets of our anti-Nestin antibody and it seems that PB9874 has been validated on mouse in both WB and IHC. Thus PB9874 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-08-15

Size

Conjugation

Total: $315

SKU:PB9874

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PB9874
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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