Anti-Nestin Antibody Picoband™

Boster Bio Anti-Nestin Antibody Picoband™ catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 24 publication(s).

Product Info Summary

SKU: PB9874
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Nestin Antibody Picoband™

See all Nestin products

SKU/Catalog Number

PB9874

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nestin Antibody Picoband™ catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nestin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9874)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nestin (252-290aa ERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAH), different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

PB9874 is reactive to NES in Human, Mouse, Rat

Applications

PB9874 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For NES (Source: Uniprot.Org, NCBI)

Uniprot ID

P48681

Gene ID

10763

Gene Name

NES

Full Name

Nestin

Weight

177.439kDa

Superfamily

intermediate filament family

Alternative Names

FLJ21841; NES; Nestin NES Nbla00170 nestin nestin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NES, check out the NES Infographic

NES infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NES: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9874 has been cited in 24 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
Species: Human

Isolation of neural crest%u2010derived stem cells from rat embryonic mandibular processes

Expression of nestin in the podocytes of normal and diseased human kidneys

Molecular and immunochemical demonstration of a novel member of Bf/C2 homolog in amphioxus Branchiostoma belcheri: implications for involvement of hepatic %u2026

Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Impaired Spatial Learning and Memory after Sevoflurane?Nitrous Oxide Anesthesia in Aged Rats Is Associated with Down-Regulated cAMP/CREB Signaling

Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells

Lentiviral-mediated growth-associated protein-43 modification of bone marrow mesenchymal stem cells improves traumatic optic neuropathy in rats

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells

Characterization and retinal neuron differentiation of WERI-Rb1 cancer stem cells

Luo L, Deng S, Yi J, Zhou S, She Y, Liu B. Evid Based Complement Alternat Med. 2017;2017:4072658. doi: 10.1155/2017/4072658. Epub 2017 Mar 8. Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neu...

Li M, Xie ZH, Yu AY, He DP. Chin Med J (Engl). 2018 Feb 5;131(3):323-329. doi: 10.4103/0366-6999.223840. Increased Efficacy of Antivenom Combined with Hyperbaric Oxygen on Deinagkistrodon acutus Envenomation in Adult Rats

Bin Wt, Ma Lm, Xu Q, Shi Xl. World J Gastroenterol. 2012 Jan 28;18(4):309-22. Doi: 10.3748/Wjg.V18.I4.309. Embryonic Hepatocyte Transplantation For Hepatic Cirrhosis: Efficacy And Mechanism Of Action.

Fu W, Lv C, Zhuang W, Chen D, Lv E, Li F, Wang X. Neural Regen Res. 2013 Feb 15;8(5):427-34. Doi: 10.3969/J.Issn.1673-5374.2013.05.006. An Effective Inducer Of Dopaminergic Neuron-Like Differentiation.

Zhang R, Pei H, Ru L, Li H, Liu G. Biomed Rep. 2013 Nov;1(6):895-900. Epub 2013 Sep 6. Bone Morphogenetic Protein 7 Upregulates The Expression Of Nestin And Glial Fibrillary Acidic Protein In Rats With Cerebral Ischemia-Reperfusion Injury.

Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.

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4 Customer Q&As for Anti-Nestin Antibody Picoband™

Question

We are currently using anti-Nestin antibody PB9874 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

The anti-Nestin antibody (PB9874) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-01

Question

My lab would like using your anti-Nestin antibody for stem cell proliferation studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

Thank you for your inquiry. This PB9874 anti-Nestin antibody is validated on hela whole cell lysates, hepg2 whole cell lysates, rat kidney tissue, mouse kidney tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-08

Question

We have seen staining in mouse cervix carcinoma. Are there any suggestions? Is anti-Nestin antibody supposed to stain cervix carcinoma positively?

C. Krishna

Verified customer

Asked: 2017-12-05

Answer

Based on literature cervix carcinoma does express NES. Based on Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414

Boster Scientific Support

Answered: 2017-12-05

Question

We have tried in the past anti-Nestin antibody for WB on metanephric glomerulus a few years ago. I am using mouse, and We intend to use the antibody for IHC next. My question regards examining metanephric glomerulus as well as neuroblastoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

K. Banerjee

Verified customer

Asked: 2017-08-15

Answer

I have checked the website and datasheets of our anti-Nestin antibody and it seems that PB9874 has been validated on mouse in both WB and IHC. Thus PB9874 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-08-15

Conjugation

how to order through PO

Size

Total: $315

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PB9874
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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