Anti-DDR2 Monoclonal Antibody

Ddr2 antibody

Boster Bio Anti-DDR2 Monoclonal Antibody catalog # M01698. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01698
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-DDR2 Monoclonal Antibody

View all Ddr2 Antibodies

SKU/Catalog Number

M01698

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DDR2 Monoclonal Antibody catalog # M01698. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01698)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACEB-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DDR2 This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01698 is reactive to DDR2 in Human, Mouse, Rat

Applications

M01698 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

v

Calculated molecular weight

96.736kDa

Background of Ddr2

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For DDR2 (Source: Uniprot.org, NCBI)

Gene Name

DDR2

Full Name

Discoidin domain-containing receptor 2

Weight

96.736kDa

Superfamily

protein kinase superfamily

Alternative Names

CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase DDR2 MIG20a, NTRKR3, TKT, TYRO10, WRCN discoidin domain receptor tyrosine kinase 2 discoidin domain-containing receptor 2|CD167 antigen-like family member B|cell migration-inducing protein 20|discoidin domain receptor 2|discoidin domain receptor family, member 2|discoidin domain-containing receptor tyrosine kinase 2|hydroxyaryl-protein kinase|migration-inducing gene 16 protein|neurotrophic tyrosine kinase receptor related 3|receptor protein-tyrosine kinase TKT|tyrosine-protein kinase TYRO10

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DDR2, check out the DDR2 Infographic

DDR2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DDR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-DDR2 Monoclonal Antibody

Question

I see that the anti-DDR2 Monoclonal antibody M01698 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-08

Question

What sample have you tested on WB/IHC with M01698?

Verified customer

Asked: 2019-12-10

Answer

For the Anti-DDR2 Monoclonal Antibody M01698, Western Blot analysis of BIRC2 expression in HepG2 cell lysate.

Boster Scientific Support

Answered: 2019-12-15

Question

My boss were well pleased with the WB result of your anti-DDR2 Monoclonal antibody. However we have observed positive staining in skin cell membrane using this antibody. Is that expected? Could you tell me where is DDR2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

From what I have seen in literature, skin does express DDR2. Generally DDR2 expresses in cell membrane. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-16

Question

Will anti-DDR2 Monoclonal antibody M01698 work for WB with hair follicle dermal papilla uterus?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

According to the expression profile of hair follicle dermal papilla uterus, DDR2 is highly expressed in hair follicle dermal papilla uterus. So, it is likely that anti-DDR2 Monoclonal antibody M01698 will work for WB with hair follicle dermal papilla uterus.

Boster Scientific Support

Answered: 2019-08-12

Question

Do you have a BSA free version of anti-DDR2 Monoclonal antibody M01698 available?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DDR2 Monoclonal antibody M01698 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-12

Question

Will M01698 anti-DDR2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

You can see on the product datasheet, M01698 anti-DDR2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-27

Question

We are currently using anti-DDR2 Monoclonal antibody M01698 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

M. Collins

Verified customer

Asked: 2019-05-03

Answer

The anti-DDR2 Monoclonal antibody (M01698) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-03

Question

See attached the WB image, lot number and protocol we used for hair follicle dermal papilla uterus using anti-DDR2 Monoclonal antibody M01698. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-24

Question

My question regards to test anti-DDR2 Monoclonal antibody M01698 on human hair follicle dermal papilla uterus for research purposes, then I may be interested in using anti-DDR2 Monoclonal antibody M01698 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-02-13

Answer

The products we sell, including anti-DDR2 Monoclonal antibody M01698, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-02-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hair follicle dermal papilla uterus using anti-DDR2 Monoclonal antibody M01698. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-17

Question

I was wanting to use your anti-DDR2 Monoclonal antibody for WB for human hair follicle dermal papilla uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hair follicle dermal papilla uterus identification?

Verified Customer

Verified customer

Asked: 2018-02-21

Answer

As indicated on the product datasheet, M01698 anti-DDR2 Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human hair follicle dermal papilla uterus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-21

Question

Is a blocking peptide available for product anti-DDR2 Monoclonal antibody (M01698)?

M. Rodriguez

Verified customer

Asked: 2018-02-07

Answer

We do provide the blocking peptide for product anti-DDR2 Monoclonal antibody (M01698). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-07

Question

My question regarding product M01698, anti-DDR2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Wu

Verified customer

Asked: 2016-02-04

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01698 anti-DDR2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-02-04

Question

We have seen staining in mouse heart thymus. Are there any suggestions? Is anti-DDR2 Monoclonal antibody supposed to stain heart thymus positively?

O. Collins

Verified customer

Asked: 2014-09-29

Answer

From literature heart thymus does express DDR2. From Uniprot.org, DDR2 is expressed in adipose tissue, heart thymus, hair follicle dermal papilla uterus, skin, cervix carcinoma, among other tissues. Regarding which tissues have DDR2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Hair follicle dermal papilla, and Uterus, Pubmed ID: 14702039
Heart, and Thymus, Pubmed ID: 8247548
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-09-29

Question

Is this M01698 anti-DDR2 Monoclonal antibody reactive to the isotypes of DDR2?

A. Zhang

Verified customer

Asked: 2014-09-18

Answer

The immunogen of M01698 anti-DDR2 Monoclonal antibody is A synthesized peptide derived from human DDR2 This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-09-18

Order DetailsPrice
M01698

100μL

$370
M01698-30ul

30μL sample

$99
M01698-carrier-free

Carrier Free

$370

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M01698
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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