Product Info Summary
SKU: | M00342 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tissue Factor Rabbit Monoclonal Antibody
View all Coagulation Factor III/Tissue Factor Antibodies
SKU/Catalog Number
M00342
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tissue Factor Rabbit Monoclonal Antibody catalog # M00342. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tissue Factor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00342)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GGE-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tissue Factor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00342 is reactive to F3 in Human, Mouse, Rat
Applications
M00342 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
33.068kDa
Background of Coagulation Factor III/Tissue Factor
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Tissue Factor expression in SHSY5Y cell lysate.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For F3 (Source: Uniprot.org, NCBI)
Gene Name
F3
Full Name
Tissue factor
Weight
33.068kDa
Superfamily
tissue factor family
Alternative Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on F3, check out the F3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for F3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tissue Factor Rabbit Monoclonal Antibody (M00342)
Hello CJ!
M00342 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Y, Cao Y, Zeng Z, Liang M, Xue Y, Xi C, Zhou M, Jiang W. Sci Rep. 2015 Feb 3;5:8209. Doi: 10.1038/Srep08209. Angiotensin-Converting Enzyme 2/Angiotensin-(1-7)/Mas Axis Prevents Lipopolysaccharide-Induced Apoptosis Of Pulmonary Microvascular End...
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