Product Info Summary
SKU: | A08779 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NEDL2 HECW2 Antibody (Internal)
SKU/Catalog Number
A08779
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NEDL2 HECW2 Antibody (Internal) catalog # A08779. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEDL2 HECW2 Antibody (Internal) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08779)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human NEDL2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08779 is reactive to HECW2 in Human
Applications
A08779 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
175.769kDa
Background of NEDL2
A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner By similarity. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Boulon S., Mol. Cell 16:777-787(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 3T3 cells using NEDL2 Polyclonal Antibody diluted at 1:1000
Click image to see more details
Western blot analysis of lysates from LOVO and HepG2 cells, using HECW2 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For HECW2 (Source: Uniprot.org, NCBI)
Gene Name
HECW2
Full Name
E3 ubiquitin-protein ligase HECW2
Weight
175.769kDa
Alternative Names
C2 and WW domain-containing protein 2; DKFZp686M17164; EC 6.3.2; EC 6.3.2.-; HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2; NEDD4-like E3 ubiquitin-protein ligase 2 HECW2 NDHSAL, NEDL2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 E3 ubiquitin-protein ligase HECW2|HECT-type E3 ubiquitin transferase HECW2|NEDD4-like E3 ubiquitin-protein ligase 2|NEDD4-related E3 ubiquitin ligase NEDL2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HECW2, check out the HECW2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HECW2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEDL2 HECW2 Antibody (Internal) (A08779)
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No publications found for A08779
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