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Product Info Summary
| SKU: | PA2023 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DISC1 Antibody Picoband®
SKU/Catalog Number
PA2023
BA2902-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DISC1 Antibody catalog # PA2023. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DISC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2023)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DISC1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2023 is reactive to DISC1 in Human
Observed Molecular Weight
22 kDa
Calculated molecular weight
93.6 kDa
Background of DISC1
DISC1 (Disrupted in Schizophrenia 1), is a protein that is encoded by the DISC1 gene in humans. Ma et al. (2002) determined that the mouse Disc1 gene maps to chromosome 8 in a region with homology of synteny to human chromosome 1q42. Ozeki et al. (2003) demonstrated that rodent Disc1 expression displayed pronounced developmental regulation, with the highest levels in late embryonic life during development of the cerebral cortex. Millar et al. (2005) showed that DISC1 interacts with the UCR2 domain of phosphodiesterase-4B, implicated in susceptibility to schizophrenia, and that elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2023 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: U87 Cell, SHG Cell,
Validation Images & Assay Conditions
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Anti-DISC1 antibody, PA2023, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: SHG Cell Lysate
Click image to see more details
Western blot analysis of DISC1 using anti-DISC1 antibody (PA2023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with anti-DISC1 antibody (PA2023) at 1:1000 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using a chemiluminescence. A specific band was detected for DISC1 at approximately 100 kDa. The expected band size for DISC1 is at 100 kDa.
Specific Publications For Anti-DISC1 Antibody Picoband® (PA2023)
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Customer Reviews
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1 Reviews For Anti-DISC1 Antibody Picoband®
The Best DISC1 Antibody on the Market--Shubhankar Nath, MOLECULAR BIOSCIENCES, UNIVERSITY OF TEXAS, GRADUATE STUDENT
Excellent
Source: Biocompare.com
| SKU | PA2023 |
|---|---|
| Applications | Western Blot |
| Sample | Jurkat cell lysate |
| Detection | Chemiluminescence |
"We tested several anti-DISC1 antibodies that are commercially available on the market. However, we found that this rabbit polyclonal anti-DISC1 antibody from Bosterbio company is the best one. We have used this antibody for IF, WB and IP purposes in NIH3T3 and Jurkat cell lines...The antibody detected a single band of protein at around 100 KD. Cheap, polyclonal, multipurpose use, fast shipping...The best DISC1 antibody in the market that can be used for IP, IF and WB."
Shubhankar Nath
Verified customer
Submitted 2016-01-19
Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-DISC1 Antibody Picoband®
Question
Would anti-DISC1 antibody PA2023 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to the expression profile of brain, DISC1 is highly expressed in brain. So, it is likely that anti-DISC1 antibody PA2023 will work for WB with brain.
Boster Scientific Support
Answered: 2019-12-18
Question
Do you have a BSA free version of anti-DISC1 antibody PA2023 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-DISC1 antibody PA2023 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
Is a blocking peptide available for product anti-DISC1 antibody (PA2023)?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We do provide the blocking peptide for product anti-DISC1 antibody (PA2023). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-11-08
Question
Will PA2023 anti-DISC1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
You can see on the product datasheet, PA2023 anti-DISC1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-05
Question
See attached the WB image, lot number and protocol we used for brain using anti-DISC1 antibody PA2023. Please let me know if you require anything else.
F. Jones
Verified customer
Asked: 2018-12-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-20
Question
I have a question about product PA2023, anti-DISC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2023 anti-DISC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-04
Question
Would anti-DISC1 antibody PA2023 work on monkey WB with buccal mucosa cell?
Verified Customer
Verified customer
Asked: 2017-06-15
Answer
Our lab technicians have not tested anti-DISC1 antibody PA2023 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-DISC1 antibody PA2023 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey buccal mucosa cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-15
