Anti-Calreticulin/CALR Antibody Picoband™

Boster Bio Anti-Calreticulin/CALR Antibody Picoband™ catalog # A00894-1. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00894-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC-P, WB

Product Name

Anti-Calreticulin/CALR Antibody Picoband™

See all Calreticulin products

SKU/Catalog Number

A00894-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Calreticulin/CALR Antibody Picoband™ catalog # A00894-1. Tested in ELISA, Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Calreticulin/CALR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00894-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Calreticulin/CALR recombinant protein (Position: E18-S323).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00894-1 is reactive to CALR in Human, Mouse, Rat

Applications

A00894-1 is guaranteed for ELISA, Flow Cytometry, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CALR (Source: Uniprot.Org, NCBI)

Uniprot ID

P27797

Gene ID

811

Gene Name

CALR

Full Name

Calreticulin

Weight

48.142kDa

Superfamily

calreticulin family

Alternative Names

Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; ERp60; FLJ26680; grp60; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; SSAHACBP; Vasostatin CALR CRT, HEL-S-99n, RO, SSA, cC1qR calreticulin calreticulin|CRP55|ERp60|HACBP|Sicca syndrome antigen A (autoantigen Ro; calreticulin)|calregulin|endoplasmic reticulum resident protein 60|epididymis secretory sperm binding protein Li 99n|grp60

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CALR, check out the CALR Infographic

CALR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CALR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-Calreticulin/CALR Antibody Picoband™

Question

To Boster Support: I am interested to purchase the Anti-Calreticulin/CALR Antibody Picoband but I need more information about it. Is the antibody could be used to detect calreticulin on cell membrane surface by flow cytometry?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

The product Anti-Calreticulin/CALR Antibody Picoband have been tested and validated to work well in Flow Cytometry with the specific species mentioned in the datasheets.

Boster Scientific Support

Answered: 2020-05-04

Question

For this product Anti-Calreticulin/CALR Antibody Picoband, does the storage buffer actually contain PBS or it is a whole ant-serum plus sodium azide?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

The product Anti-Calreticulin/CALR Antibody Picoband formulation is 100

Boster Scientific Support

Answered: 2020-05-01

Question

Our lab used your anti-Calreticulin/CALR antibody for ELISA on brain a few years ago. I am using mouse, and We intend to use the antibody for WB next. I am interested in examining brain as well as keratinocyte in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

I have checked the website and datasheets of our anti-Calreticulin/CALR antibody and it seems that A00894-1 has been validated on mouse in both ELISA and WB. Thus A00894-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-16

Question

We want using your anti-Calreticulin/CALR antibody for sequestering of calcium ion studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

Z. Baker

Verified customer

Asked: 2019-11-15

Answer

Thanks for your inquiry. This A00894-1 anti-Calreticulin/CALR antibody is tested on human placenta tissue, hepg2 whole cell lysates. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-15

Question

We were happy with the WB result of your anti-Calreticulin/CALR antibody. However we have been able to see positive staining in colon carcinoma endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is CALR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-12

Answer

Based on literature, colon carcinoma does express CALR. Generally CALR expresses in endoplasmic reticulum lumen. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874

Boster Scientific Support

Answered: 2019-11-12

Question

We are currently using anti-Calreticulin/CALR antibody A00894-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

The anti-Calreticulin/CALR antibody (A00894-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-20

Question

We have observed staining in human thyroid gland. Any tips? Is anti-Calreticulin/CALR antibody supposed to stain thyroid gland positively?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

Based on literature thyroid gland does express CALR. Based on Uniprot.org, CALR is expressed in thyroid gland, eye, pancreas skin, placenta, liver, brain, cajal-retzius cell fetal brain cortex, keratinocyte, colon carcinoma, among other tissues. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874

Boster Scientific Support

Answered: 2019-09-11

Size

Total: $280

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

In stock
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A00894-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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