Anti-Dishevelled 3/DVL3 Antibody Picoband™

Boster Bio Anti-Dishevelled 3/DVL3 Antibody Picoband™ catalog # A03577. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03577
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Dishevelled 3/DVL3 Antibody Picoband™

See all Dishevelled-3 products

SKU/Catalog Number

A03577

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Dishevelled 3/DVL3 Antibody Picoband™ catalog # A03577. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Dishevelled 3/DVL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03577)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Dishevelled 3 (397-434aa DTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMW L), identical to the related mouse sequence.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A03577 is reactive to DVL3 in Human, Mouse, Rat

Applications

A03577 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For DVL3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q92997

Gene ID

1857

Gene Name

DVL3

Full Name

Segment polarity protein dishevelled homolog DVL-3

Weight

78.055kDa

Superfamily

DSH family

Alternative Names

dishevelled 3 (homologous to Drosophila dsh); dishevelled, dsh homolog 3 (Drosophila); Dishevelled3; Dishevelled-3; DSH homolog 3; DVL3; KIAA0208dishevelled-3; segment polarity protein dishevelled homolog DVL-3 DVL3 DRS3 dishevelled segment polarity protein 3 segment polarity protein dishevelled homolog DVL-3|dishevelled 3 (homologous to Drosophila dsh)|dishevelled, dsh homolog 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DVL3, check out the DVL3 Infographic

DVL3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DVL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-Dishevelled 3/DVL3 Antibody Picoband™

Question

Is this A03577 anti-Dishevelled 3/DVL3 antibody reactive to the isotypes of DVL3?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

The immunogen of A03577 anti-Dishevelled 3/DVL3 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Dishevelled 3 (397-434aa DTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMW L), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-22

Question

My question regarding product A03577, anti-Dishevelled 3/DVL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03577 anti-Dishevelled 3/DVL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-10

Question

I have attached the WB image, lot number and protocol we used for brain using anti-Dishevelled 3/DVL3 antibody A03577. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-28

Question

We are currently using anti-Dishevelled 3/DVL3 antibody A03577 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

The anti-Dishevelled 3/DVL3 antibody (A03577) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-22

Question

I was wanting to use to test anti-Dishevelled 3/DVL3 antibody A03577 on mouse brain for research purposes, then I may be interested in using anti-Dishevelled 3/DVL3 antibody A03577 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-09-20

Answer

The products we sell, including anti-Dishevelled 3/DVL3 antibody A03577, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-09-20

Question

Does anti-Dishevelled 3/DVL3 antibody A03577 work for WB with brain?

R. Parker

Verified customer

Asked: 2013-07-05

Answer

According to the expression profile of brain, DVL3 is highly expressed in brain. So, it is likely that anti-Dishevelled 3/DVL3 antibody A03577 will work for WB with brain.

Boster Scientific Support

Answered: 2013-07-05

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A03577
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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