Anti-Calpain-6 CAPN6 Antibody

Product Name

Anti-Calpain-6 CAPN6 Antibody

View all CAPN6 Antibodies

SKU/Catalog Number

A09778

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Calpain-6 CAPN6 Antibody (Catalog # A09778). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

CAPN6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Calpain-6 CAPN6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09778)

Host

Rabbit

Contents

CAPN6 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CAPN6 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human CAPN6. The immunogen is located within the last 50 amino acids of CAPN6.

Reactive Species

A09778 is reactive to CAPN6 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

74.6 kDa

Background of CAPN6

Calpains make up a ubiquitously expressed, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. This large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 (CAPN6) is most similar to Calpain 5; the C-terminal region of CAPN6 lacks homology to the calmodulin-like domain of other vertebrate calpains. CAPN6 is thought to be involved in the regulation of microtubule dynamics and cytoskeletal organization. CAPN6 has also been recently identified as an HIV dependency factor (HDF), suggesting that CAPN6 may be an important drug target in HIV treatment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09778 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CAPN6 antibody can be used for the detection of StrepII by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CAPN6

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