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Product Info Summary
| SKU: | A04214 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-DNMT3L Antibody
SKU/Catalog Number
A04214
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-DNMT3L Antibody (Catalog # A04214). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-DNMT3L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04214)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
Reactive Species
A04214 is reactive to DNMT3L in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.6 kDa
Background of DNMT3L
DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04214 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1000 - 1:3000
IHC: 1:500
IP: 1:100
WB: 1:500
Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide. This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation. The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.
Validation Images & Assay Conditions
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Affinity Purified Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4µg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL.
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The conserved N-terminal cysteine-rich region, the PHD-like motif, characteristic of the Dnmt3 family is shown. The C-terminal domain is the catalytic methyltransferase domain present in Dnmt3a and Dnmt3b whereas the key catalytic motifs are absent in Dnmt3L. Numbers indicate amino acid residues.
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Immunohistochemistry Rabbit Anti-Human DNMT3L Antibody. Antibody: Rabbit Anti-Human DNMT3L Antibody at 1:500. Antigen Retrieval: HIER pH 6.2 Staining: Nuclear staining of testicular germ cells (Left); Neg Ctrl (Right) Human testis 40X.
Specific Publications For Anti-DNMT3L Antibody (A04214)
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Customer Q&As
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12 Customer Q&As for Anti-DNMT3L Antibody
Question
Is a blocking peptide available for product anti-DNMT3L antibody (A04214)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-DNMT3L antibody (A04214). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
Please see the WB image, lot number and protocol we used for placenta using anti-DNMT3L antibody A04214. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-10
Question
We are interested in to test anti-DNMT3L antibody A04214 on human placenta for research purposes, then I may be interested in using anti-DNMT3L antibody A04214 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The products we sell, including anti-DNMT3L antibody A04214, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-18
Question
Will A04214 anti-DNMT3L antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Zhao
Verified customer
Asked: 2018-10-09
Answer
It shows on the product datasheet, A04214 anti-DNMT3L antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-09
Question
Does anti-DNMT3L antibody A04214 work on zebrafish WB with placenta?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Our lab technicians have not validated anti-DNMT3L antibody A04214 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-DNMT3L antibody A04214 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-03
Question
I see that the anti-DNMT3L antibody A04214 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-05
Question
I have a question about product A04214, anti-DNMT3L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Lewis
Verified customer
Asked: 2017-05-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04214 anti-DNMT3L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-23
Question
I was wanting to use your anti-DNMT3L antibody for WB for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
A. Carter
Verified customer
Asked: 2015-08-21
Answer
It shows on the product datasheet, A04214 anti-DNMT3L antibody has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-08-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-DNMT3L antibody A04214. Let me know if you need anything else.
Z. Thomas
Verified customer
Asked: 2014-10-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-06
Question
We are currently using anti-DNMT3L antibody A04214 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
P. Jackson
Verified customer
Asked: 2014-09-04
Answer
The anti-DNMT3L antibody (A04214) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-04
Question
Will anti-DNMT3L antibody A04214 work for WB with placenta?
A. Krishna
Verified customer
Asked: 2014-06-25
Answer
According to the expression profile of placenta, DNMT3L is highly expressed in placenta. So, it is likely that anti-DNMT3L antibody A04214 will work for WB with placenta.
Boster Scientific Support
Answered: 2014-06-25
Question
Do you have a BSA free version of anti-DNMT3L antibody A04214 available?
P. Carter
Verified customer
Asked: 2013-11-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DNMT3L antibody A04214 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-28
