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Product Info Summary
| SKU: | PB9936 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Calcitonin/CALCA Antibody
SKU/Catalog Number
PB9936
PB0966 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calcitonin/CALCA Antibody catalog # PB9936. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calcitonin/CALCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9936)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Calcitonin, different from the related mouse and rat sequences by four amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9936 is reactive to CALCA in Human
Calculated molecular weight
13.9 kDa
Background of CALCA
Calcitonin, also known as CALCA, is a peptide hormone synthesized by the parafollicular cells of the thyroid. It is mapped to 11p15.2. Calcitonin belongs to the calcitonin-like protein family. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9936 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
IHC: human lung cancer tissue, human thyroid cancer tissue
Validation Images & Assay Conditions
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IHC analysis of CGRP using anti-CGRP antibody (PB9936).
CGRP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CGRP Antibody (PB9936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of CGRP using anti-CGRP antibody (PB9936).
CGRP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CGRP Antibody (PB9936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Calcitonin/CALCA Antibody (PB9936)
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15 Customer Q&As for Anti-Calcitonin/CALCA Antibody
Question
you antibody to test anti-Calcitonin/CALCA antibody PB9936 on human pheochromocytoma for research purposes, then I may be interested in using anti-Calcitonin/CALCA antibody PB9936 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The products we sell, including anti-Calcitonin/CALCA antibody PB9936, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-30
Question
I was wanting to use your anti-Calcitonin/CALCA antibody for IHC for human pheochromocytoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pheochromocytoma identification?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
You can see on the product datasheet, PB9936 anti-Calcitonin/CALCA antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human pheochromocytoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
Is this PB9936 anti-Calcitonin/CALCA antibody reactive to the isotypes of CALCA?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The immunogen of PB9936 anti-Calcitonin/CALCA antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Calcitonin (83-119aa ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAF), different from the related mouse and rat sequences by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-14
Question
Does anti-Calcitonin/CALCA antibody PB9936 work on primate IHC with pheochromocytoma?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
Our lab technicians have not tested anti-Calcitonin/CALCA antibody PB9936 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Calcitonin/CALCA antibody PB9936 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pheochromocytoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-Calcitonin/CALCA antibody (PB9936)?
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
We do provide the blocking peptide for product anti-Calcitonin/CALCA antibody (PB9936). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-03-25
Question
My question regarding product PB9936, anti-Calcitonin/CALCA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9936 anti-Calcitonin/CALCA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-27
Question
I see that the anti-Calcitonin/CALCA antibody PB9936 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-24
Question
Is there a BSA free version of anti-Calcitonin/CALCA antibody PB9936 available?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Calcitonin/CALCA antibody PB9936 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-12
Question
We are currently using anti-Calcitonin/CALCA antibody PB9936 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
T. Carter
Verified customer
Asked: 2017-12-12
Answer
The anti-Calcitonin/CALCA antibody (PB9936) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-12
Question
We have observed staining in human thyroid carcinoma. What should we do? Is anti-Calcitonin/CALCA antibody supposed to stain thyroid carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
Based on literature thyroid carcinoma does express CALCA. Based on Uniprot.org, CALCA is expressed in dorsal root ganglion, thyroid carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2017-07-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pheochromocytoma using anti-Calcitonin/CALCA antibody PB9936. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-28
Question
Would PB9936 anti-Calcitonin/CALCA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Li
Verified customer
Asked: 2015-12-11
Answer
It shows on the product datasheet, PB9936 anti-Calcitonin/CALCA antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-11
Question
See attached the WB image, lot number and protocol we used for pheochromocytoma using anti-Calcitonin/CALCA antibody PB9936. Please let me know if you require anything else.
M. Yang
Verified customer
Asked: 2015-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-15
Question
Our team were happy with the WB result of your anti-Calcitonin/CALCA antibody. However we have observed positive staining in thyroid carcinoma secreted. using this antibody. Is that expected? Could you tell me where is CALCA supposed to be expressed?
C. Miller
Verified customer
Asked: 2015-07-01
Answer
From what I have seen in literature, thyroid carcinoma does express CALCA. Generally CALCA expresses in secreted. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2015-07-01
Question
Would anti-Calcitonin/CALCA antibody PB9936 work for IHC with pheochromocytoma?
D. Miller
Verified customer
Asked: 2014-09-17
Answer
According to the expression profile of pheochromocytoma, CALCA is highly expressed in pheochromocytoma. So, it is likely that anti-Calcitonin/CALCA antibody PB9936 will work for IHC with pheochromocytoma.
Boster Scientific Support
Answered: 2014-09-17
