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Product Info Summary
| SKU: | M00840-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-DOT1L Mouse Monoclonal Antibody [Clone ID: OTI1D8]
SKU/Catalog Number
M00840-1
Size
100 µl
Description
Boster Bio DOT1L mouse monoclonal antibody, clone OTI1D8 (formerly 1D8). Catalog# M00840-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-DOT1L Mouse Monoclonal Antibody [Clone ID: OTI1D8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00840-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1D8
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-434 of human DOT1L (NP_115871) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00840-1 is reactive to DOT1L in Human
Reconstitution
Calculated molecular weight
164.856kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00840-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Protein Target Info & Infographic
Gene/Protein Information For DOT1L (Source: Uniprot.org, NCBI)
Gene Name
DOT1L
Full Name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
Weight
164.856kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4 DOT1L DOT1, KMT4 DOT1 like histone lysine methyltransferase histone-lysine N-methyltransferase, H3 lysine-79 specific|DOT1 like histone H3K79 methyltransferase|DOT1-like histone methyltransferase|DOT1-like protein|DOT1-like, histone H3 methyltransferase|H3-K79-HMTase|histone H3-K79 methyltransferase|histone methyltransferase DOT1L|lysine N-methyltransferase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DOT1L, check out the DOT1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DOT1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DOT1L Mouse Monoclonal Antibody [Clone ID: OTI1D8] (M00840-1)
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