DOT1L Antibodies

AND DOT1L ELISA kits, DOT1L Proteins

Many biological assays use antibodies to detect DOT1L, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DOT1L in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DOT1L antibodies...

We validate the specificity of these antibodies to DOT1L by testing them on tissues known to express DOT1L positively and negatively. Browse below to find the DOT1L antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for DOT1L? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the DOT1L Western Blot Guide

DOT1L Infographic by Boster Bio

All DOT1L Products

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Applications

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Anti-KMT4 / Dot1L Rabbit Monoclonal Antibody

Western blot analysis of KMT4/Dot1L expression in RAW264

IHC analysis of DOT1L using anti-DOT1L antibody (M00840)

Human, Mouse

IHC, WB

$370
Cat # M00840
100 μl

DOT1L (NM_032482) Human Over-expression Lysate

Human

$960
Cat # LS084444
20 µg, 100 µg

DOT1L Overview

Facts about Histone-lysine N-methyltransferase, H3 lysine-79 specific.

DOT1L 3D structure. Source: alphafold

Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L)

Histone methyltransferase. Methylates 'Lys-79' of histone H3.

Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582). Binds to DNA (PubMed:12628190).

Gene Information

Human
Gene Name:	DOT1L
Uniprot:	Q8TEK3
Entrez:	84444

Family

Belongs to:

class I-like SAM-binding methyltransferase superfamily

Alternative Names

DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4

Molecular Weight

Mass (kDA):
164.856 kDA

Genomic Context


Human

Location:	19p13.3
Sequence:	19; NC_000019.10 (2163933..2232578)

Subcellular Localizations

Nucleus.

Diseases

Leukemia
Malignant Neoplasms
Leukemogenesis
Chromosomal Translocation
Cell Transformation, Neoplastic
Neoplasms
Leukemia, Myelocytic, Acute
Myeloid Leukemia
Acute Leukemia
Leukemia, Experimental
Acute Lymphocytic Leukemia
Aneuploidy
Precursor T-cell Lymphoblastic Leukemia-lymphoma

Precursor B-cell Lymphoblastic Leukemia-lymphoma
Glomerulosclerosis (disorder)
Cytogenetic Abnormality
Kidney Diseases
Diabetic Nephropathy
Ataxia
Pancytopenia

Interacting Proteins

MLLT1
MLLT3

Pathways

Methylation
Cell Cycle
Histone Methylation
Histone Modification
Transport
Nuclear Export
Localization
Cell Cycle Checkpoint
Regulation Of Gene Expression
Demethylation
Rna Processing
Protein Phosphorylation
Gene Silencing

Brain Development
Aging
Dna Demethylation
Liver Development
Cell Cycle Arrest
Histone Phosphorylation
Reverse Transcription

PTMs

Methylation
Phosphorylation
Demethylation

Reduction
Acetylation
Bromination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DOT1L

References

PMID: 12123582 by Feng Q., et al. Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain.

PMID: 12693554 by Jikuya H., et al. Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones.