Anti-Protein yippee-like 5 YPEL5 Antibody

Product Name

Anti-Protein yippee-like 5 YPEL5 Antibody

View all YPEL5 Antibodies

SKU/Catalog Number

A15188

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Protein yippee-like 5 YPEL5 Antibody (Catalog # A15188). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

YPEL5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Protein yippee-like 5 YPEL5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15188)

Host

Rabbit

Contents

YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5. The immunogen is located within the first 50 amino acids of YPEL5.

Reactive Species

A15188 is reactive to YPEL5 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

13.8 kDa

Background of YPEL5

YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A15188 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information YPEL5

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